AML1-ETO antibody - ChIP grade

Cat. No. GTX60339

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ELISA, ChIP assay
Reactivity	Human
Package	50 μl ($399)

See all AML1-ETO products

Datasheet

View product citations for antibody GTX60339 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1,000
ELISA	1:500
ChIP assay	1-10 μl

Not tested in other applications.

Product Note

This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/ Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22 (t(8;21)(q22;q22)).

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The AML1-ETO fusion protein using a KLH-conjugated synthetic peptide.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

runt related transcription factor 1 , RUNX1 translocation partner 1 , AML1 , AML1-EVI-1 , AMLCR1 , CBF2alpha , CBFA2 , EVI-1 , PEBP2aB , PEBP2alpha , AML1-MTG8 , AML1T1 , CBFA2T1 , CDR , ETO , MTG8 , ZMYND2

Cellular Localization

Nucleus

Database

Gene ID: 861 RUNX1
Gene ID: 862 RUNX1T1
UniProt: Q01196 RUNX1
UniProt: Q06455 RUNX1T1

Research Area

DATA IMAGES

GTX60339 WB Image

WB analysis of nuclear extracts of SKNO-1 cells (15 μg) using GTX60339 AML1-ETO antibody - ChIP grade.
Dilution : 1:1,000

GTX60339 ELISA Image

ELISA analysis of peptides used for immunization using GTX60339 AML1-ETO antibody - ChIP grade.

GTX60339 ChIP assay Image

ChIP analysis of sheared chromatin from 1.25x10⁶ Kasumi-1 cells using GTX60339 AML1-ETO antibody - ChIP grade. QPCR was performed using primers specific for the FUT7, NFE2 and OGG1 genes. This figure shows the occupancy, calculated as the ratio + control/background for which the promoter of the H2B gene was used.
Antibody amount : 4μl

REFERENCE

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REVIEW

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Package

List Price ($)

50 μl

$ 399

Qty