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APE1 antibody

KO/KD
Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in Immunoprecipitation (IP). GTX110558
Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110558
Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in Western Blot (WB). GTX110558
Anti-APE1 antibody used in ChIP assay (ChIP assay). GTX110558
Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110558

Cat. No. GTX110558

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all APE1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

36 kDa. ( Note )

Positive Control

PC-12 , mouse brain , HeLa

Predict Reactivity

Bovine, Dog, Pig, Chimpanzee, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human APE1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949671

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

apurinic/apyrimidinic endodeoxyribonuclease 1 , APE , APE1 , APEN , APEX , APX , HAP1 , REF1

Cellular Localization

Nucleus

Background

Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-APE1 antibody used in Western Blot (WB). GTX110558

GTX110558 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-APE1 antibody used in Immunoprecipitation (IP). GTX110558

GTX110558 IP Image

APE1 antibody immunoprecipitates APE1 protein in IP experiments. IP Sample: A431 whole cell lysate/extract A : 30 μg whole cell lysate/extract of APE1 protein expressing A431 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of APE1 by 3 μg of APE1 antibody (GTX110558) 10% SDS-PAGE The immunoprecipitated APE1 protein was detected by APE1 antibody (GTX110558) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-APE1 antibody used in Western Blot (WB). GTX110558

GTX110558 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-APE1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110558

GTX110558 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A549, using APE1(GTX110558) antibody at 1:200 dilution.

Anti-APE1 antibody used in Western Blot (WB). GTX110558

GTX110558 WB Image

APE1 antibody detects APEX1 protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
10% SDS-PAGE
APE1 antibody (GTX110558) dilution: 1:10000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-APE1 antibody used in Western Blot (WB). GTX110558

GTX110558 WB Image

APE1 antibody detects APE1 protein by Western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with APE1 antibody (GTX110558) diluted by 1:1000.

Anti-APE1 antibody used in ChIP assay (ChIP assay). GTX110558

GTX110558 ChIP assay Image

Cross-linked ChIP was performed with 293T chromatin extract treated with Trichostatin A (0.4 μM for 18 h) and 5 μg of either control rabbit IgG or anti-APE1 antibody. The precipitated DNA was detected by PCR with primer set targeting to MDR1 promoter.
ChIP experiment and primer designs are based on Oncogene. 2011 Jan 27;30(4):482-93.

Anti-APE1 antibody used in IHC (Paraffin sections) (IHC-P). GTX110558

GTX110558 IHC-P Image

APE1 antibody detects APE1 protein at cytoplasm, nucleus and nucleolus on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
APE1 antibody (GTX110558) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Verena Hurst et al. Mol Biol Cell 2021; 32 (20) : br6 Cytoskeleton integrity influences XRCC1 and PCNA dynamics at DNA damage.
Application : ICC/IF
Reactivity : Human
Ken Asada et al. Nucleic Acids Res 2021; 49 (9) : 4864-4876 Short DNA/RNA heteroduplex oligonucleotide interacting proteins are key regulators of target gene silencing.
Application : WB
Reactivity : Mouse
Martins MB et al. Mutagenesis 2021; 36 (3) : 223-236 NEK1 deficiency affects mitochondrial functions and the transcriptome of key DNA repair pathways.
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169