Caspase 1 antibody [N1N3]

Name: Caspase 1 antibody [N1N3]
Brand: GeneTex
SKU: GTX101322
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-Caspase 1 antibody [N1N3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101322

Anti-Caspase 1 antibody [N1N3] used in IHC (Paraffin sections) (IHC-P). GTX101322

Anti-Caspase 1 antibody [N1N3] used in Western Blot (WB). GTX101322

Cat. No. GTX101322

Reference 6

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Caspase 1 products

View product citations for antibody GTX101322 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:100-1:1000

Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

THP-1

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Caspase 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10618781

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

caspase 1 , ICE , IL1BC , P45

Cellular Localization

Cytoplasm

Background

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. This gene was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. This gene has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing of this gene results in five transcript variants encoding distinct isoforms. [provided by RefSeq]

Database

Gene ID: 834 CASP1
UniProt: P29466 CASP1

Research Area

DATA IMAGES

GTX101322 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using Caspase 1 alpha(GTX101322) antibody at 1:500 dilution.

GTX101322 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using Caspase 1 alpha(GTX101322) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101322 IHC-P Image

Caspase 1 antibody [N1N3] detects Caspase 1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Caspase 1 stained by Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX101322 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Caspase 1 antibody [N1N3] (GTX101322) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX101322 IHC-P Image

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660

GTX101322 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2013. PMID: 23653660

REFERENCE

Application Reference

Hosoya T et al. J Am Soc Nephrol 2022; 33 (2) : 326-341 Xanthine Oxidoreductase Inhibitors Suppress the Onset of Exercise-Induced AKI in High HPRT Activity Urat1-Uox Double Knockout Mice.

Application : WB

Reactivity : Mouse

Feng M et al. Int J Mol Sci 2022; 23 (17) Anti-Inflammation and Anti-Pyroptosis Activities of Mangiferin via Suppressing NF-κB/NLRP3/GSDMD Signaling Cascades.

Application : WB

Reactivity : Mouse

Zhang C et al. Mol Med Rep 2021; 23 (5) Cytokine secretion and pyroptosis of cholesteatoma keratinocytes mediated by AIM2 inflammasomes in response to cytoplasmic DNA.

Application : WB, IHC-P

Reactivity : Human

Wu CY et al. J Cell Mol Med 2020; (Epub) Tris DBA ameliorates IgA nephropathy by blunting the activating signal of NLRP3 inflammasome through SIRT1- and SIRT3-mediated autophagy induction.

Application : WB

Reactivity : Mouse

Chen C.Y. et al. Clinical Science 2018; 132 (20) : 2221-2239 Omega-3 Polyunsaturated Fatty Acids Reduce Preterm Labor by Inhibiting Trophoblast Cathepsin S and Inflammasome Activation.

Application : WB

Reactivity : Human

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