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DNA Polymerase I (E coli) antibody

Anti-DNA Polymerase I (E coli) antibody used in Western Blot (WB). GTX64108
Anti-DNA Polymerase I (E coli) antibody used in Immunoprecipitation (IP). GTX64108

Cat. No. GTX64108

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 E. coli
Package
100 μl ($399)
See all DNA Polymerase I products

APPLICATION

Application Note

Western blot. (1:1,000-1:5,000 dilution)

PROPERTIES

Form

Liquid

Buffer

Serum

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

E. coli

Immunogen

Full-size recombinant E. coli DNA polymerase 1.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

Anti-DNA Polymerase I (E coli) antibody used in Western Blot (WB). GTX64108

GTX64108 WB Image

Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using anti-DNA polymerase 1 antibody. 1; Purified E. coli DNA polymerase 1 (10 ng) 2; Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1/2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa.

Anti-DNA Polymerase I (E coli) antibody used in Immunoprecipitation (IP). GTX64108

GTX64108 IP Image

Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with anti-DNA polymerase 1 antibody 1. Purified DNA polymerase 1 ( 20 ng), WB 2. Purified DNA polymerase 1 (10 ng), WB 3. Crude lysate of E. coli strain AB1157 (10 μg), WB 4. The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP for IP was used.

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399