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E-Cadherin antibody

KO/KD Comparison Orthogonal
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

Cat. No. GTX100443

Reference 111
8

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm, IP, PLA

Reactivity

 Human, Mouse, Rat, Zebrafish, Dog
Package
100 μl ($399),
25 μl ($169)
See all E-Cadherin products
PRODUCT

Summary

E-Cadherin antibody detects E-cadherin protein, which is encoded by the CDH1 gene. E-cadherin is a calcium-dependent cell adhesion protein (predicted molecular weight of 97 kDa) that plays a vital role in cell migration and proliferation. Because E-cadherin is an epithelial cell marker, this antibody is a well-published and widely used reagent to study E-cadherin protein expression during the epithelial-mesenchymal transition (EMT) in various physiological and pathological processes.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
IP 1:100-1:500
PLA Assay dependent
Not tested in other applications.

Calculated MW

97 kDa. ( Note )

Positive Control

MCF-7

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.14 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human E-Cadherin. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_10729586

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

cadherin 1 , Arc-1 , BCDS1 , CD324 , CDHE , ECAD , LCAM , UVO

Cellular Localization

Cell junction , Cell membrane

Background

This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function is thought to contribute to progression in cancer by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. Identified transcript variants arise from mutation at consensus splice sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with E-Cadherin antibody (GTX100443) diluted at 1:3000 and competitor's antibody diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

KO/KD

Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat duodenum.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog skin.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443

GTX100443 IP Image

E-cadherin antibody immunoprecipitates E-cadherin protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 3 μg of preimmune Rabbit IgG
B. Immunoprecipitation of E-cadherin protein by 3 μg E-cadherin antibody (GTX100443)
5 % SDS-PAGE
The immunoprecipitated E-cadherin protein was detected by E-cadherin antibody (GTX100443) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: HCT116 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using E-Cadherin antibody (GTX100443).

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-cadherin antibody detects E-cadherin protein at membrane on human breast cancer by immunohistochemical analysis.
Sample: Paraffin-embedded breast cancer.
E-cadherin antibody (GTX100443) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat prostate by immunohistochemical analysis.
Sample: Paraffin-embedded rat prostate.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse pancreas by immunohistochemical analysis.
Sample: Paraffin-embedded mouse pancreas.
E-Cadherin antibody (GTX100443) diluted at 1:400.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
E-Cadherin antibody (GTX100443) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin protein stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-E-Cadherin antibody used in Immunoprecipitation (IP). GTX100443

GTX100443 IP Image

Immunoprecipitation of E-Cadherin protein from MCF-7 whole cell extracts using 5 μg of E-Cadherin antibody (GTX100443).
Western blot analysis was performed using E-Cadherin antibody (GTX100443).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with E-Cadherin antibody (GTX100443) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443

GTX100443 ICC/IF Image

E-Cadherin antibody detects E-Cadherin protein at cell membrane by immunofluorescent analysis.
Sample: MDCK cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: E-Cadherin stained by E-Cadherin antibody (GTX100443) diluted at 1:500.

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Orthogonal

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

Orthogonal

The data was published in the journal Front Oncol in 2020.PMID: 32211324

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28683123

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal PLoS One in 2017. PMID: 28683123

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28195146

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Front Endocrinol (Lausanne) in 2017. PMID: 29109696

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35517780

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

The data was published in the journal Cell Death Dis in 2020.PMID: 32080178

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Int J Mol Sci in 2019.PMID: 31426484

Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443

GTX100443 IHC-P Image

The data was published in the journal Evid Based Complement Alternat Med in 2019. PMID: 31186661

Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443

GTX100443 WB Image

The data was published in the journal Evid Based Complement Alternat Med in 2019. PMID: 31186661

REFERENCE

Application Reference

Pecci V et al. Cancer Cell Int 2024; 24 (1) : 56 Targeting of H19/cell adhesion molecules circuitry by GSK-J4 epidrug inhibits metastatic progression in prostate cancer.
Phan QT et al. PLoS Pathog 2023; 19 (8) : e1011579 Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.
Chen WC et al. Cancers (Basel) 2023; 15 (15) Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic Profiling.
Yin L et al. J Exp Clin Cancer Res 2023; 42 (1) : 204 AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer.
Application : WB
Reactivity : Human
TH Hung et al. Biomed J 2023; : 100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
Application : WB
Reactivity : Human
Show More +
Submit a Reference

REVIEW

E-Cadherin antibody Cat. No. GTX100443

Rating
( Average 4.8 based on 8 users reviews)
Application
Western Blot(WB)
( Average 4.7 based on 6 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-E-Cadherin antibody used in IHC (Paraffin sections) (IHC-P). GTX100443
Date : Anonymous submitted on 10-Oct-2023
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse Brain tissue
Blocking : 0.5% skim milk in PBST
Fixation : 4% PFA
Permeabilization /Antigen retrieval : PBS-T / Microwave heating in citrate buffer
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Date : Anonymous submitted on 27-Jan-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells, MCF-7 cells
Amount used : 30μg
Blocking : 5% non-fat milk in PBS, 4°C, 1.5Hr
Primary Antibody : 1:3000, 4°C, > 12Hr
Anti-E-Cadherin antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100443
Date : Anonymous submitted on 22-Sep-2021
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : A549 cells
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, RT, 0.5Hr
Primary Antibody : 1:100, 4°C, 16Hr
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Date : Anonymous submitted on 22-Feb-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : colonic mucosa
Amount used : 25 μg
Blocking : 5% fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Date : Anonymous submitted on 30-Dec-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : A549 cells
Amount used : 80 μg
Blocking : 1% skim milk, 24°C, 1Hr
Primary Antibody : 1:2000, 24°C, 2Hr
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Date : Anonymous submitted on 21-Jun-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : oecm1 cells
Amount used : 20 μg
Blocking : 10% non-fat milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 24Hr
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: hFOB1.19 cells, Lane 2: MG63 cells, Lane 3: U2OS cells
Anti-E-Cadherin antibody used in Western Blot (WB). GTX100443
Date : Anonymous submitted on 24-Apr-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : HT-29 cells
Amount used : 30μg
Blocking : 5% skim milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Show More +
Submit a Review
Application Reference
Pecci V et al. Cancer Cell Int 2024; 24 (1):56 Targeting of H19/cell adhesion molecules circuitry by GSK-J4 epidrug inhibits metastatic progression in prostate cancer.
Phan QT et al. PLoS Pathog 2023; 19 (8):e1011579 Candida albicans stimulates formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.
Chen WC et al. Cancers (Basel) 2023; 15 (15) Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic Profiling.
Yin L et al. J Exp Clin Cancer Res 2023; 42 (1):204 AR antagonists develop drug resistance through TOMM20 autophagic degradation-promoted transformation to neuroendocrine prostate cancer.
Application : WB
Reactivity : Human
TH Hung et al. Biomed J 2023;:100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
Application : WB
Reactivity : Human
Wan-Ping Su et al. Biomed Pharmacother 2023; 163 Boosting mitochondrial function and metabolism in aging female germ cells with dual ROCK/ROS inhibition
Application : WB
Reactivity : Human
Chang TM et al. Int J Biol Sci 2023; 19 (5):1455-1470 CXCL14 promotes metastasis of non-small cell lung cancer through ACKR2-depended signaling pathway.
Application : WB, ICC/IF
Reactivity : Human
Wu CH et al. Cancers (Basel) 2023; 15 (5)
Application : WB
Reactivity : Human
Lin YC et al. iScience 2023; 26 (3):106089 CAR-T cells targeting HLA-G as potent therapeutic strategy for EGFR-mutated and overexpressed oral cancer.
Application : WB
Reactivity : Human
Chiou HC et al. Antioxidants (Basel) 2023; 12 (2) Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human.
Application : WB
Reactivity : Human
Zhi W et al. J Med Virol 2023; 95 (1):e28009 HPV-CCDC106 integration promotes cervical cancer progression by facilitating the high expression of CCDC106 after HPV E6 splicing.
Application : ICC/IF
Reactivity : Human
Phan QT et al. bioRxiv 2023; Candida albicans stimulates the formation of a multi-receptor complex that mediates epithelial cell invasion during oropharyngeal infection.
Application : PLA
Su CC et al. Nutrients 2023; 15 (23) Protective Effect of Alpha-Linolenic Acid on Human Oral Squamous Cell Carcinoma Metastasis and Apoptotic Cell Death.
Masola V et al. Front Oncol 2022; 12 (0):918419 Heparanase regulates EMT and cancer stem cell properties in prostate tumors.
Application : WB
Reactivity : Human
Kosim MY et al. Front Pharmacol 2022; 13:988335 p53 status modifies cytotoxic activity of lactoferrin under hypoxic conditions.
Application : WB
Reactivity : Human
Wang L et al. Bioengineered 2022; 13 (5):12941-12954 Stearoyl-CoA desaturase 1 regulates malignant progression of cervical cancer cells.
Ying Liu et al. J Cancer 2022; 13 (7):2258-2270 PlexinA1 activation induced by β2-AR promotes epithelial-mesenchymal transition through JAK-STAT3 signaling in human gastric cancer cells.
Application : ICC/IF
Reactivity : Human
Huang-Chi Chen et al. Front Pharmacol 2022; 13:877125 Effects of Montelukast on Arsenic-Induced Epithelial-Mesenchymal Transition and the Role of Reactive Oxygen Species Production in Human Bronchial Epithelial Cells.
Application : WB
Reactivity : Human
Xiaoyan Wang et al. Front Immunol 2022; 13:864984 MicroRNA-382 Promotes M2-Like Macrophage via the SIRP-α/STAT3 Signaling Pathway in Aristolochic Acid-Induced Renal Fibrosis.
Application : WB
Reactivity : Mouse
Zou W et al. J Cell Mol Med 2022; siRNA-induced CD44 knockdown suppresses the proliferation and invasion of colorectal cancer stem cells through inhibiting epithelial-mesenchymal transition.
Application : WB
Reactivity : Mouse
Arciniega-Mart?nez IM et al. Mol Med Rep 2022; 25 (2) CD4+/IL?4+ lymphocytes of the lamina propria and substance P promote colonic protection during acute stress.
Application : WB
Reactivity : Mouse
Lee WJ et al. J Food Drug Anal 2021; 29 (1):113-127 Tea polyphenol epigallocatechin-3-gallate inhibits cell proliferation in a patient-derived triple-negative breast cancer xenograft mouse model via inhibition of proline-dehydrogenase-induced effects.
Ting-Ting Yen et al. Sci Rep 2021; 11 (1):21737
Application : WB, IHC-P
Reactivity : Human, Mouse
Aoshima Y et al. Am J Transl Res 2021; 13 (11):12318-12337
Application : IHC-P
Reactivity : Mouse
Ciarpella F et al. iScience 2021; 24 (12):103438 Murine cerebral organoids develop network of functional neurons and hippocampal brain region identity.
Application : IHC-P
Reactivity : Mouse
Gaur G et al. Antibiotics (Basel) 2021; 10 (11) Effect of Invasion of Borrelia burgdorferi in Normal and Neoplastic Mammary Epithelial Cells.
Application : WB
Petrella C et al. Pharmacol Res 2021; 172:105795 Proneurogenic and neuroprotective effect of a multi strain probiotic mixture in a mouse model of acute inflammation: Involvement of the gut-brain axis.
Application : WB
Reactivity : Mouse
Kawami M et al. Toxicology 2021; 461:152903 Evaluation on epithelial-mesenchymal state and microRNAs focusing on isolated alveolar epithelial cells from bleomycin injured rat lung.
Application : WB
Reactivity : Rat
Kawaguchi K et al. iScience 2021; 24 (9):103022 Cellular senescence promotes cancer metastasis by enhancing soluble E-cadherin production.
Application : WB
Reactivity : Mouse
Lai YW et al. Cells 2021; 10 (8) Prognostic Value of a Glycolytic Signature and Its Regulation by Y-Box-Binding Protein 1 in Triple-Negative Breast Cancer.
Application : WB
Reactivity : Human
Wang LT et al. Cancer Lett 2021; 520:160-171 Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance.
Application : WB
Reactivity : Mouse
Nicolai A et al. Pharmaceuticals (Basel) 2021; 14 (6) Anti-Tumoral Effects of a (1H-Pyrrol-1-yl)Methyl-1H-Benzoimidazole Carbamate Ester Derivative on Head and Neck Squamous Carcinoma Cell Lines.
Application : WB
Reactivity : Human
Wang J et al. Cancer Manag Res 2021; 13:2701-2715 Circular RNA_0000629 Suppresses Bladder Cancer Progression Mediating MicroRNA-1290/CDC73.
Application : WB
Reactivity : Human
Lv F et al. Eur J Pharmacol 2021; 900:174069 Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway.
Li Y et al. Transl Lung Cancer Res 2021; 10 (4):1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Hu W et al. JCI Insight 2021; 6 (3) NOGOB receptor-mediated RAS signaling pathway is a target for suppressing proliferating hemangioma.
Application : WB
Reactivity : Mouse
Chen H.Y. et al. Cancers 2021; 13 (6):1236 Isoliquiritigenin Reverses Epithelial-Mesenchymal Transition Through Modulation of the TGF-β/Smad Signaling Pathway in Endometrial Cancer.
Application : WB
Reactivity : Human
Li G et al. J Cell Sci 2021; 134 (2) Matrix stiffness regulates α-TAT1-mediated acetylation of α-tubulin and promotes silica-induced epithelial-mesenchymal transition via DNA damage.
Application : WB
Reactivity : Human
Kuo TT et al. ACS Omega 2020; 5 (49):31792-31800 Melissa officinalis Extract Induces Apoptosis and Inhibits Migration in Human Colorectal Cancer Cells.
Application : WB
Reactivity : Human
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
Application : WB
Reactivity : Human
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
Application : WB
Reactivity : Human
Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
Application : WB
Reactivity : Human
Cao C et al. Front Oncol 2020; 10:284 LOXL2 Expression Status Is Correlated With Molecular Characterizations of Cervical Carcinoma and Associated With Poor Cancer Survival via Epithelial-Mesenchymal Transition (EMT) Phenotype.
Wang J et al. Onco Targets Ther 2020; 13:9333-9348
Application : WB
Reactivity : Human
Pan CM et al. Cancer Lett 2020; 493:133-142 MicroRNA-7 targets T-Box 2 to inhibit epithelial-mesenchymal transition and invasiveness in glioblastoma multiforme.
Application : WB
Reactivity : Human
Yamazaki M et al. Lab Invest 2020; (Epub) Continuous formation of small clusters with LGR5-positive cells contributes to tumor growth in a colorectal cancer xenograft model.
Application : IHC-P
Reactivity : Human
Lee JY et al. PLoS One 2020; 15 (7):e0236876 PHRF1 promotes migration and invasion by modulating ZEB1 expression.
Application : WB
Reactivity : Human
Crespo ?C et al. Cell 2020; 182 (5):1125-1139.e18 Decidual NK Cells Transfer Granulysin to Selectively Kill Bacteria in Trophoblasts.
Application : ICC/IF
Reactivity : Mouse
Gaballa R et al. Cancers (Basel) 2020; 12 (8) Exosomes-Mediated Transfer of Itga2 Promotes Migration and Invasion of Prostate Cancer Cells by Inducing Epithelial-Mesenchymal Transition.
Application : WB
Reactivity : Human
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
Application : WB
Reactivity : Human
Wang SC et al. Biochem Pharmacol 2020; 180:114152 α-Linolenic acid inhibits the migration of human triple-negative breast cancer cells by attenuating Twist1 expression and suppressing Twist1-mediated epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Bray JK et al. Cell Death Dis 2020; 11 (2):138 Loss of RE-1 silencing transcription factor accelerates exocrine damage from pancreatic injury.
Application : IHC-P
Reactivity : Mouse
Yang P et al. Exp Biol Med (Maywood) 2019; 244 (18):1608-1618 ResolvinD1 attenuates high-mobility group box 1-induced epithelial-to-mesenchymal transition in nasopharyngeal carcinoma cells.
Yang P et al. Int J Med Sci 2019; 16 (12):1631-1641 Agonist of PPAR-γ Reduced Epithelial-Mesenchymal Transition in Eosinophilic Chronic Rhinosinusitis with Nasal Polyps via Inhibition of High Mobility Group Box1.
Palazzolo S et al. Cancers (Basel) 2019; 11 (12) An Effective Multi-Stage Liposomal DNA Origami Nanosystem for In Vivo Cancer Therapy.
Application : IHC-P
Reactivity : Mouse
Terasaki M et al. J Clin Med 2019; 9 (1) Dietary Fucoxanthin Induces Anoikis in Colorectal Adenocarcinoma by Suppressing Integrin Signaling in a Murine Colorectal Cancer Model.
Application : WB
Reactivity : Mouse
Kim H et al. Micromachines (Basel) 2019; 10 (11) Mechanical Property Changes in Breast Cancer Cells Induced by Stimulation with Macrophage Secretions in Vitro.
Application : ICC/IF
Reactivity : Mouse
Zhang L et al. Cells 2019; 8 (10) Estimating Dynamic Cellular Morphological Properties via the Combination of the RTCA System and a Hough-Transform-Based Algorithm.
Application : WB
Reactivity : Human
Zhang X et al. Evid Based Complement Alternat Med 2019; 2019:5370847 Qingshen Buyang Formula Attenuates Renal Fibrosis in 5/6 Nephrectomized Rats via Inhibiting EMT and Wnt/帣-Catenin Pathway.
Jill B. Graham et al. bioRxiv 2019; (Epub) ER transmembrane protein TMTC3 contributes to O-mannosylation of E-cadherin, Cellular Adherence and Embryonic Gastrulation.
Application : WB
Reactivity : Human
Tsai CH et al. Biochim Biophys Acta Mol Cell Res 2019; 1867 (2):118564 Aza-PBHA, a potent histone deacetylase inhibitor, inhibits human gastric-cancer cell migration via PKC帢-mediated AHR-HDAC interactions.
Application : WB
Reactivity : Human
Hsu SY et al. Onco Targets Ther 2019; 12:7611-7621 A novel CXCL8 analog is effective in inhibiting the growth via cell cycle arrest and attenuating invasion of Lewis lung carcinoma.
Application : WB
Reactivity : Mouse
Bacci L et al. Int J Mol Sci 2019; 20 (16) H19-Dependent Transcriptional Regulation of 帣3 and 帣4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.
Application : WB
Reactivity : Human
Lixia Yang et al. European Journal of Inflammation 2019; (Epub) Effects of total flavonoids from Oxytropis falcata Bunge on the SOCS/JAK/STAT inflammatory signaling pathway in the kidneys of diabetic nephropathy model mice.
Application : WB
Reactivity : Mouse
Li-Chien Chang et al. Nutrition 2019; (Epub) 1,25(OH)2D3 attenuates indoxyl sulfate-induced epithelial-to-mesenchymal cell transition via inactivation of PI3K/Akt/?]-catenin signaling in renal tubular epithelial cells.
Chen KY et al. PLoS One 2019; 14 (7):e0219317 Resveratrol induced premature senescence and inhibited epithelial-mesenchymal transition of cancer cells via induction of tumor suppressor Rad9.
Application : WB
Reactivity : Human
Shukla A et al. JCI Insight 2019; 5 Self-reactive B cells in the GALT are actively curtailed to prevent gut inflammation.
Application : WB
Reactivity : Human
Su KJ et al. Cancers (Basel) 2019; 11 (8) Downregulation of SHIP2 by Hepatitis B Virus X Promotes the Metastasis and Chemoresistance of Hepatocellular Carcinoma through SKP2.
Application : WB
Reactivity : Human
Liu J et al. Mol Med Rep 2019; 20 (1):33-40 miR?338?3p inhibits A549 lung cancer cell proliferation and invasion by targeting AKT and β?catenin signaling pathways.
Application : ICC/IF
Reactivity : Human
Xueqin Zhang et al. Hindawi 2019; (Epub) Qingshen Buyang Formula Attenuates Renal Fibrosis in 5/6 Nephrectomized Rats via Inhibiting EMT and Wnt/β-Catenin Pathway.
Application : IHC-P
Reactivity : Rat
Chu CA et al. EBioMedicine 2019; 43:270-281 MiR-338-5p promotes metastasis of colorectal cancer by inhibition of phosphatidylinositol 3-kinase, catalytic subunit type 3-mediated autophagy pathway.
Application : WB
Reactivity : Human
Ahsan K et al. Dev Biol 2019; 448 (1):16-35 Prickle1 is required for EMT and migration of zebrafish cranial neural crest.
Application : IHC-Wm
Reactivity : Zebrafish
Ma C et al. Onco Targets Ther 2019; 12:1475-1495 Sympathetic innervation contributes to perineural invasion of salivary adenoid cystic carcinoma via the β2-adrenergic receptor.
Application : WB
Reactivity : Human
Zhong C et al. Oncol Lett 2018; 16 (2):1455-1462 12-Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial-mesenchymal transition.
Chang CY et al. Cell Death Discov 2018; 4:100 PEGylated liposome-encapsulated rhenium-188 radiopharmaceutical inhibits proliferation and epithelial-mesenchymal transition of human head and neck cancer cells in vivo with repeated therapy.
Application : WB
Reactivity : Mouse
DaSilva-Arnold S et al. Mol Hum Reprod 2018; (Epub) heZEB2, a master regulator of the epithelial-mesenchymal transition, mediates trophoblast differentiation.
Application : WB
Reactivity : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
Application : WB
Reactivity : Human
Masaru Terasaki et al. J. Clin. Biochem. Nutr. 2018;:43107 Fucoxanthin administration delays occurrence of tumors in xenograft mice by colonospheres, with an anti-tumor predictor of glycine.
Application : WB
Reactivity : Human
Rusdamayanti Rusdamayanti et al. Indonesian Journal of Pathology 2018; 27:2 Ekspresi E-cadherin dan MMP-1 pada Adenokarsinoma Kolorek-tal dengan dan Tanpa Metastasis KGB.
Application : IHC-P
Zhong C et al. Oncology Letters 2018; (Epub) 12-Lipoxygenase promotes invasion and metastasis of human gastric cancer cells via epithelial-mesenchymal transition.
Application : IHC-P
Reactivity : Human
Nguyen QH et al. Nat Commun 2018; 9 (1):2028 Profiling human breast epithelial cells using single cell RNA sequencing identifies cell diversity.
Application : IHC-P
Reactivity : Human
Terasaki M et al. Anticancer Res 2018; 38 (4):2169-2179 Glycine Is a Predictor for a Suppressive Effect of Fucoxanthinol on Colonosphere Formation Under Hypoxia.
Application : WB
Reactivity : Human
Wei Q et al. Mol Med Rep 2018; (Epub) Effects of bradykinin on TGF-β1-induced epithelial-mesenchymal transition in ARPE-19 cells.
Application : WB
Reactivity : Human
DaSilva-Arnold S et al. Biol Reprod 2018; (Epub) Human trophoblast epithelial-mesenchymal transition in abnormally invasive placenta.
Application : WB
Reactivity : Human
Yang H et al. Int J Mol Med 2017; 40 (5):1466-1476 Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD.
Amador C. V. et al. J Clin Exp Pathol 2017; 7:6 MUC1 and C-Met Affects Proliferation, Intercellular Junctions and Invasion in Two Head and Neck Carcinoma Cell Lines.
Application : WB, ICC/IF
Reactivity : Human
Chen PC et al. J Cell Physiol 2017; (Epub) Activation of Fibroblasts by Nicotine Promotes the Epithelial-Mesenchymal Transition and Motility of Breast Cancer Cells.
Application : WB
Reactivity : Human
Chamard-Jovenin C et al. Front Endocrinol (Lausanne) 2017; 8:272 Low-Dose Alkylphenol Exposure Promotes Mammary Epithelium Alterations and Transgenerational Developmental Defects, But Does Not Enhance Tumorigenic Behavior of Breast Cancer Cells.
Application : ICC/IF
Reactivity : Human
Taglieri L et al. Invest New Drugs 2017; (Epub) The kinesin Eg5 inhibitor K858 induces apoptosis and reverses the malignant invasive phenotype in human glioblastoma cells.
Application : WB
Reactivity : Human
Yang H et al. International Journal of Molecular Medicine 2017; (Epub) Runx3 is a key modulator during the epithelial-mesenchymal transition of alveolar type II cells in animal models of BPD.
Application : WB
Reactivity : Rat
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
Application : WB
Lee AF et al. PLoS One 2017; 12 (7):e0180383 Reverse epithelial-mesenchymal transition contributes to the regain of drug sensitivity in tyrosine kinase inhibitor-resistant non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Chao CC et al. FEBS Lett 2017; (Epub) Downregulation of miR-199a/b-5p is associated with GCNT2 induction upon epithelial-mesenchymal transition in colon cancer.
Application : WB
Reactivity : Human
Xu F et al. Cancer Cell Int 2017; 17:54 Hypoxia and TGF-β1 induced PLOD2 expression improve the migration and invasion of cervical cancer cells by promoting epithelial-to-mesenchymal transition (EMT) and focal adhesion formation.
Application : WB
Reactivity : Human
Su NW et al. Molecules 2017; 22 (4) Metronomic Cordycepin Therapy Prolongs Survival of Oral Cancer-Bearing Mice and Inhibits Epithelial-Mesenchymal Transition.
Application : IHC-P
Reactivity : Human
Hsu LS et al. Int J Med Sci 2017; 14 (6):530-535 KLF6 inhibited oral cancer migration and invasion via downregulation of mesenchymal markers and inhibition of MMP-9 activities.
Application : WB
Reactivity : Human
Su D et al. Onco Targets Ther 2017; 10:1549-1559 Knockdown of IQGAP1 inhibits proliferation and epithelial-mesenchymal transition by Wnt/β-catenin pathway in thyroid cancer.
Application : WB
Reactivity : Human
Chen X et al. Tumour Biol 2017; 39 (3):1.01E+15 Sulfiredoxin may promote metastasis and invasion of cervical squamous cell carcinoma by epithelial-mesenchymal transition.
Application : WB, IHC-P
Reactivity : Human
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
Application : WB
Reactivity : Human
Thiebaut C et al. PLoS One 2017; 12 (3):e0173931 Mammary epithelial cell phenotype disruption in vitro and in vivo through ERalpha36 overexpression.
Application : WB, ICC/IF
Reactivity : Mouse
Tsai CH et al. Sci Rep 2017; 7:41927 The inhibition of lung cancer cell migration by AhR-regulated autophagy.
Application : WB
Reactivity : Human
Li CH et al. Arch Toxicol 2016; (Epub) Cytoplasmic aryl hydrocarbon receptor regulates glycogen synthase kinase 3 beta, accelerates vimentin degradation, and suppresses epithelial-mesenchymal transition in non-small cell lung cancer cells.
Application : WB
Reactivity : Human
Sridaran D et al. Tumour Biol 2016; Oxystressed tumor microenvironment potentiates epithelial to mesenchymal transition and alters cellular bioenergetics towards cancer progression.
Application : WB
Reactivity : Human
Zhao B et al. J Proteomics 2015; 112:38-52 Comprehensive proteome quantification reveals NgBR as a new regulator for epithelial-mesenchymal transition of breast tumor cells.
Application : WB
Reactivity : Human
Wang TH et al. Oncotarget 2015; 6 (27):23342-57 Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition.
Application : WB
Reactivity : Human
Chen CY et al. Oncotarget 2015; 6 (29):26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
Application : WB
Reactivity : Human
Sun Y et al. Int J Clin Exp Pathol 2015; 8 (10):12775-83 miR-204 inhibits invasion and epithelial-mesenchymal transition by targeting FOXM1 in esophageal cancer.
Application : WB
Reactivity : Human
Yurong Sun et al. Int J Clin Exp Pathol 2015; 8 (10):12775-12783 miR-204 inhibits invasion and epithelial-mesenchymal transition by targeting FOXM1 in esophageal cancer.
Application : WB
Reactivity : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
Application : WB
Reactivity : Human
Lupia A et al. J Invest Dermatol 2014; 134 (12):2947-56 CD63 tetraspanin is a negative driver of epithelial-to-mesenchymal transition in human melanoma cells.
Application : WB
Reactivity : Human
Li X et al. Lab Chip 2014; 14 (3):471-81 Micro-scaffold array chip for upgrading cell-based high-throughput drug testing to 3D using benchtop equipment.
Application : ICC/IF
Reactivity : Human
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