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Ebola virus GP1 antibody

Anti-Ebola virus GP1 antibody used in Western Blot (WB). GTX134144
Anti-Ebola virus GP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134144

Cat. No. GTX134144

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Ebola virus
Package
100 μl ($399),
25 μl ($169)
See all Ebola virus GP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Positive Control

Zaire ebolavirus GP-transfected 293T

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.67 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Ebola virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Ebola virus GP1 (Zaire ebolavirus (strain Kikwit-95)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887233

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Ebola virus GP protein , EBOV GP protein

Research Area

DATA IMAGES

Anti-Ebola virus GP1 antibody used in Western Blot (WB). GTX134144

GTX134144 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Ebola virus GP1 antibody (GTX134144) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Ebola virus GP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134144

GTX134144 ICC/IF Image

Ebola virus GP1 antibody detects Ebola virus GP1 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ebola virus GP1 protein stained by Ebola virus GP1 antibody (GTX134144) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Mai Izumida et al. Viruses 2022; 14 (4) Unique Mode of Antiviral Action of a Marine Alkaloid against Ebola Virus and SARS-CoV-2.
Pham-Tue-Hung Tran et al. Microorganisms 2020; 8 (12) : E1890 Development of a Multivalent Kunjin Virus Reporter Virus-Like Particle System Inducing Seroconversion for Ebola and West Nile Virus Proteins in Mice.
Application : WB, ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169