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Enterovirus D68 VP1 antibody [GT1843]

Orthogonal
Anti-Enterovirus D68 VP1 antibody [GT1843] used in Lateral Flow (Lateral Flow). GTX633770
Anti-Enterovirus D68 VP1 antibody [GT1843] used in Lateral Flow (Lateral Flow). GTX633770
Anti-Enterovirus D68 VP1 antibody [GT1843] used in Western Blot (WB). GTX633770
Anti-Enterovirus D68 VP1 antibody [GT1843] used in Western Blot (WB). GTX633770
Anti-Enterovirus D68 VP1 antibody [GT1843] used in ELISA (ELISA). GTX633770
Anti-Enterovirus D68 VP1 antibody [GT1843] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633770
Anti-Enterovirus D68 VP1 antibody [GT1843] used in ELISA (ELISA). GTX633770
Anti-Enterovirus D68 VP1 antibody [GT1843] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX633770

Cat. No. GTX633770

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT1843

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, ELISA, Lateral Flow, IHC-P (cell pellet)

Reactivity

 Enterovirus D68
Package
100 μl ($399),
25 μl ($169)
See all Enterovirus D68 VP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

Lateral Flow
Capture: GTX633770, Detection: GTX637898 or Capture: GTX637898, Detection: GTX633770
Not tested in other applications.

Product Note

This antibody was raised against Enterovirus D68 VP1, and it does not cross-react with Enterovirus 71 VP1.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Enterovirus D68

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of Enterovirus D68 VP1 protein. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888374

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Enterovirus D68 Capsid protein VP1 , Enterovirus D68 VP1 , EVD68 Capsid protein VP1 , EVD68 VP1

Research Area

DATA IMAGES

Anti-Enterovirus D68 VP1 antibody [GT1843] used in Lateral Flow (Lateral Flow). GTX633770

GTX633770 Lateral Flow Image

Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Enterovirus D68 VP1 antibody (GTX633770 [GT1843])
Detection: Enterovirus D68 VP1 antibody (GTX637898 [HL1997])

Samples (80 ng) :
1. Enterovirus D68 VP1 protein (GTX138561-pro)
2. Lysis buffer

Anti-Enterovirus D68 VP1 antibody [GT1843] used in Lateral Flow (Lateral Flow). GTX633770

GTX633770 Lateral Flow Image

Detection of enterovirus D68 VP1 protein by lateral flow assay using the monoclonal antibody pair.

Capture: Enterovirus D68 VP1 antibody (GTX637898 [HL1997])
Detection: Enterovirus D68 VP1 antibody (GTX633770 [GT1843])

Samples (80 ng) :
1. Enterovirus D68 VP1 protein (GTX138561-pro)
2. Lysis buffer

Anti-Enterovirus D68 VP1 antibody [GT1843] used in Western Blot (WB). GTX633770

GTX633770 WB Image

Non-infected (–) and infected (+) RD whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus D68 VP1 antibody [GT1843] used in Western Blot (WB). GTX633770

GTX633770 WB Image

Orthogonal

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Enterovirus D68 VP1 antibody [GT1843] used in ELISA (ELISA). GTX633770

GTX633770 ELISA Image

Orthogonal

Sandwich ELISA detection of recombinant full-length VP1 protein(s) derived from different strains of Enterovirus (ie., D68; 71) using antibodies as below.

Capture: Enterovirus D68 VP1 antibody [GT1843] (GTX633770) (5 μg/mL)
Detection: Enterovirus D68 VP1 antibody [HL1997] (GTX637898) (1 μg/mL)

Anti-Enterovirus D68 VP1 antibody [GT1843] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633770

GTX633770 ICC/IF Image

Enterovirus D68 VP1 antibody [GT1843] detects Enterovirus D68 VP1 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Enterovirus D68 VP1 antibody [GT1843] used in ELISA (ELISA). GTX633770

GTX633770 ELISA Image

Indirect ELISA analysis was performed by coating the plate with recombinant full-length enterovirus D68 VP1 (4000-62.5 ng/mL). Coated protein was probed with Enterovirus D68 VP1 antibody [GT1843] (GTX633770) (1 μg/mL). Goat anti-mouse IgG antibody (HRP) (GTX213111-01) (1:10000) was used to detect the bound primary antibody.

Anti-Enterovirus D68 VP1 antibody [GT1843] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX633770

GTX633770 IHC-P (cell pellet) Image

Enterovirus D68 VP1 antibody [GT1843] detects Enterovirus D68 VP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mock and Enterovirus D68 VP1 transfected 293T cell.
Green: Enterovirus D68 VP1 stained by Enterovirus D68 VP1 antibody [GT1843] (GTX633770) diluted at 1:4000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

Application Reference

Vogt MR et al. J Pediatric Infect Dis Soc 2018; 7 (suppl_2) : S49-S53 Current Understanding of Humoral Immunity to Enterovirus D68.
Zheng HW et al. Viruses 2017; 9 (5) Nasal Infection of Enterovirus D68 Leading to Lower Respiratory Tract Pathogenesis in Ferrets (Mustela putorius furo).
Application : IHC-P
Submit a Reference

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169