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IRF3 antibody [5D2]

KO/KD Overexpression
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287
Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287
Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287

Cat. No. GTX84287

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5D2

Isotype

 IgG2b

Application

 WB, IHC-P

Reactivity

 Human, Monkey
Package
100 μl ($519)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human IRF3 expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm,Nucleus

Background

Mediates interferon-stimulated response element (ISRE) promoter activation. Functions as a molecular switch for antiviral activity. DsRNA generated during the course of an viral infection leads to IRF3 phosphorylation on the C-terminal serine/threonine cluster. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of genes under the control of ISRE. The complex binds to the IE and PRDIII regions on the IFN-alpha and IFN-beta promoters respectively. IRF-3 does not have any transcription activation domains.

Database

Research Area

DATA IMAGES

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of thyroid tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of breast tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of endometrium tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of bladder carcinoma tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287

GTX84287 WB Image

Overexpression

WB analysis of HEK293T cells transfected with IRF3 plasmid (Right) or empty vector (Left) for 48 hrs using GTX84287 IRF3 antibody [5D2].
Loading : 5 ug per lane

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of ovary tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287

GTX84287 WB Image

WB analysis of various cell lines using GTX84287 IRF3 antibody [5D2].
Loading : 35 ug per lane

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of lung tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in IHC (Paraffin sections) (IHC-P). GTX84287

GTX84287 IHC-P Image

IHC-P analysis of pancreas tissue using GTX84287 IRF3 antibody [5D2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287

GTX84287 WB Image

KO/KD

WB analysis of wild type and IRF3 knock out HeLa cell lysate(10μg per lane) using GTX84287 IRF3 antibody [5D2].
Dilution : 1:500

Anti-IRF3 antibody [5D2] used in Western Blot (WB). GTX84287

GTX84287 WB Image

The data was published in the 2022 in Front Pharmacol. PMID: 35308231

REFERENCE

Application Reference

Xue Zhang et al. Front Pharmacol 2022; 13 : 827128 A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo.
Application : WB
Reactivity : Human
Submit a Reference

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519