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IRF3 (phospho Ser386) antibody

Orthogonal
Anti-IRF3 (phospho Ser386) antibody used in Western Blot (WB). GTX130422
Anti-IRF3 (phospho Ser386) antibody used in Western Blot (WB). GTX130422
Anti-IRF3 (phospho Ser386) antibody used in Western Blot (WB). GTX130422

Cat. No. GTX130422

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all IRF3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.04 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser386 of human IRF3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886261

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

interferon regulatory factor 3 , IIAE7

Cellular Localization

Cytoplasm , Nucleus

Background

IRF3 encodes interferon regulatory factor 3, a member of the interferon regulatory transcription factor (IRF) family. IRF3 is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-IRF3 (phospho Ser386) antibody used in Western Blot (WB). GTX130422

GTX130422 WB Image

Orthogonal

Untreated (–) and treated (+) THP-1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF3 (phospho Ser386) antibody (GTX130422) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-IRF3 (phospho Ser386) antibody used in Western Blot (WB). GTX130422

GTX130422 WB Image

Orthogonal

IRF3 (phospho Ser386) antibody detects IRF3 (phospho Ser386) protein by western blot analysis. Un-treated (-) and treated (+, CIP treatment for 1hr) HeLa whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with IRF3 (phospho Ser386) antibody (GTX130422) at a dilution of 1:5000.

Anti-IRF3 (phospho Ser386) antibody used in Western Blot (WB). GTX130422

GTX130422 WB Image

Orthogonal

The data was published in the 2022 in Front Pharmacol. PMID: 35308231

REFERENCE

Application Reference

WC Chiou et al. Biomed Pharmacother 2023; 170 : 116077 Ergosterol peroxide blocks HDV infection as a novel entry inhibitor by targeting human NTCP receptor
Xue Zhang et al. Front Pharmacol 2022; 13 : 827128 A Polysaccharide From Eupolyphaga sinensis Walker With Anti-HBV Activities In Vitro and In Vivo.
Application : WB
Reactivity : Human
Jia Z Shen et al. Cell 2020; S0092-8674 (20) : 31614-7 FBXO44 promotes DNA replication-coupled repetitive element silencing in cancer cells.
Application : WB
Reactivity : Human
Soonthornvacharin S et al. Nat Microbiol 2017; 2 : 17022 Systems-based analysis of RIG-I-dependent signalling identifies KHSRP as an inhibitor of RIG-I receptor activation.
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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169