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JNK (phospho Thr183/Tyr185) antibody

Orthogonal
Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX24821
Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24821

Cat. No. GTX24821

Reference 14

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, IHC

Reactivity

 Human, Mouse, Rat, Pig, Primate
Package
50 μl ($399)
See all JNK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200-1:2000
ICC/IF 1:250
IHC-P Assay dependent
IP Assay dependent
IHC Assay dependent
Not tested in other applications.

Product Note

This antibody is raised against JNK1/2 phosphorylated at Thr183/Tyr185. Based on sequence homology, it is predicted to react with JNK3 when phosphorylated at the corresponding residues - Thr221/Tyr223. The antibody has been shown to recognize the endogenous, active forms of JNK 1 + 2 in a variety of cell types following treatment by a broad range of extracellular stimuli [e.g. including 293 cells (human embryonic kidney; +/- ultraviolet light) and PC12 cells (rat pheochromocytoma; +/- sorbital)].

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA, 50% Glycerol

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against a chemically synthesized phosphopeptide derived from a region of human JNK1 and 2 that contains threonine 183 and tyrosine 185. This region is conserved among many species including mouse, rat, chicken, nematode, fruit fly, and in JNK3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_369523

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Cellular oncogenes, or proto-oncogenes, play pivotal roles in cellular communication pathways that regulate normal growth, development and differentiation. The cellular oncogene families fos and jun encode nuclear proteins that can function as transcription factors. The fos family of nuclear oncogenes encode cFos, FosB, (fos-related antigen) Fra1, and Fra2. The jun family member, cJun, is phosphorylated by another kinase termed cJun N-terminal kinase (JNK).

Research Area

DATA IMAGES

Anti-JNK (phospho Thr183/Tyr185) antibody used in Western Blot (WB). GTX24821

GTX24821 WB Image

Orthogonal

WB analysis of various samples using GTX24821 JNK (phospho Thr183/Tyr185) antibody.
Dilution : 1:1000
Lane 1: HEK-293
Lane 2: HEK-293 treated for 5 minutes with 200 mM of Anisomycin
Lane 3: HEK-293 treated for 20 minutes with UV
Lane 4: MCF7
Lane 5: MCF7 treated for 5 minutes with 200 mM of Anisomycin
Lane 6: K562
Lane 7: K562 treated for 20 minutes with UV
Lane 8: HeLa
Lane 9: HeLa treated for 20 minutes with UV
Loading : 20ug

Anti-JNK (phospho Thr183/Tyr185) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24821

GTX24821 ICC/IF Image

Orthogonal

ICC/IF analysis of A549 cells treated with Anisomycin (25 μg/mL for 30 min) using GTX24821 JNK (phospho Thr183/Tyr185) antibody. Panel e is untreated cell with no signal. Panel f is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.25% Triton X-100 for 10 minutes

REFERENCE

Application Reference

Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Sun Y et al. Immun Inflamm Dis 2022; 10 (7) : e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Chang JW et al. Cereb Cortex 2020; 30 (8) : 4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Taisuke Okano et al. International Journal of Endocrinology 2020; 2020 : Article ID 4139093 β-Endorphin Mediates the Development and Instability of Atherosclerotic Plaques
Application : WB
Reactivity : Human
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REVIEW

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Application Reference
Lee SC et al. Pharmaceuticals (Basel) 2023; 16 (9) Synergistic Effect of Ginsenoside Rh2 Combines with Ionizing Radiation on CT26/luc Colon Carcinoma Cells and Tumor-Bearing Animal Model.
Sun Y et al. Immun Inflamm Dis 2022; 10 (7):e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
Huang CY et al. Nutrients 2020; 12 (6) Attenuation of Lipopolysaccharide-Induced Acute Lung Injury by Hispolon in Mice, Through Regulating the TLR4/PI3K/Akt/mTOR and Keap1/Nrf2/HO-1 Pathways, and Suppressing Oxidative Stress-Mediated ER Stress-Induced Apoptosis and Autophagy.
Application : WB
Reactivity : Mouse
Chang JW et al. Cereb Cortex 2020; 30 (8):4597-4606 Ultrasound Stimulation Suppresses LPS-Induced Proinflammatory Responses by Regulating NF-κB and CREB Activation in Microglial Cells.
Application : WB
Reactivity : Rat
Taisuke Okano et al. International Journal of Endocrinology 2020; 2020:Article ID 4139093 β-Endorphin Mediates the Development and Instability of Atherosclerotic Plaques
Application : WB
Reactivity : Human
Wu CT et al. Oxid Med Cell Longev 2019; 2019:9056845 Salvianolic Acid C against Acetaminophen-Induced Acute Liver Injury by Attenuating Inflammation, Oxidative Stress, and Apoptosis through Inhibition of the Keap1/Nrf2/HO-1 Signaling.
Application : WB
Reactivity : Mouse
Jau?Tien Lin et al. Journal of Food Biochemistry 2019; (Epub) Litchi (Litchi chinensis Sonn.) flower proanthocyanidin fraction exhibited protective efficacy to suppress nickel?induced expression for vascular endothelial growth factor in HepG2 cells.
Application : WB
Reactivity : Human
Hsieh WY et al. PLoS One 2019; 14 (1):e0210656 Renal chymase-dependent pathway for angiotensin II formation mediated acute kidney injury in a mouse model of aristolochic acid I-induced acute nephropathy.
Application : WB
Reactivity : Mouse
Lin JT et al. Journal of Functional Foods 2018; 47:343-349 Protective effect and mechanism of Muntingia calabura Linn. fruit ethanolic extract against vascular endothelial growth factor production in nickel-stimulated hepatocellular carcinoma cells.
Application : WB
Reactivity : Human
Segawa R et al. J Dermatol Sci 2017; (Epub) EGFR transactivation is involved in TNF-α-induced expression of thymic stromal lymphopoietin in human keratinocyte cell line.
Application : WB
Reactivity : Human
Yeh CJ et al. Sci Rep 2017; 7 (1):15599 The effects of artocarpin on wound healing: in vitro and in vivo studies.
Application : WB
Reactivity : Human
Lin WC et al. RSC Adv. 2017; 7:21234-21251 Anti-inflammatory activity of Sanghuangporus sanghuang by suppressing the TLR4-mediated PI3K/AKT/mTOR/IKKβ signaling pathway.
Application : WB
Reactivity : Mouse
Lee YR et al. J Biomed Sci 2012; 19:9 Reversine suppresses oral squamous cell carcinoma via cell cycle arrest and concomitantly apoptosis and autophagy.
Application : WB
Reactivity : Human
Yan MY et al. Int J Mol Med 2012; 29 (5):855-63 Tanshinone IIA inhibits BT-20 human breast cancer cell proliferation through increasing caspase 12, GADD153 and phospho-p38 protein expression.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399