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Methylated cytosine DNA standard kit

Methylated cytosine DNA standard kit. GTX400004
Methylated cytosine DNA standard kit. GTX400004

Cat. No. GTX400004

Reference 1

Application

 Dot
Package
1 kit ($299)
See all 5-hmC, 5-mC & cytosine products
PRODUCT

Summary

The Methylated cytosine DNA standard kit is a set of five DNA standards that are linear dsDNA, 426 bp, and have the same sequence (see sequence below & figure). The only difference is that each contains either normal cytosines, 5-methylcytosines, 5-hydroxymethylcytosines, 5-formylcytosines, or 5-carboxylcytosines. Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.
Sequence Information:
5'CGGGGTACCTTCACTTCAGAATCAACCAAACAGCCAAAACTGTTACATCAGGTTGTGGA GCAGTTACAAAAGGTTCATTTTATCACAGATACCCTGTCAAAGGGTGAGACAAAGTTCATG GGTGTTTGCCAGCTTCCCAGTAAAAATGATGAAAAAGAATATCCACACAGAAGAATTGATA TCAGGTTGATACCCAAAGATCAGTATTACTGTGGTGTTCTCTATTTCACTGGGAGTGATAT TTTCAATAAGAATATGAGGGCTCATGCCCTAGAAAAGGGTTTCACAATCAATGAGTACACC ATCCGTCCCTTGGGAGTCACTGGAGTTGCAGGAGAACCCCTGCCAGTGGATAGTGAAAAA GACATCTTTGATTACATCCAGTGGAAATACCGGGAACCCAAGGACCGGAGCGAAGAATTC CCG 3'
Note: Sequence is same for all five standards. Depending on the DNA, all C’s are unmodified cytosine, 5-methylcytosine, 5-hydroxymethylcytosine, 5-formylcytosine, or 5-carboxylcytosine.

Sequence statistics:

Nucleotide Count Frequency (%)
Adenine (A) 138 32.4 %
Thymine (T) 103 24.2 %
Cytosine (C) 88 20.7 %
Guanine (G) 97 22.8 %
C+G 185 43.4 %
A+T 241 56.6 %

Content

Content Package Storage
Cytosine DNA Standard 2 μg 4ºC or below
5-Methylcytosine DNA Standard 2 μg 4ºC or below
5-Hydroxymethylcytosine DNA Standard 2 μg 4ºC or below
5-Formylcytosine DNA Standard 2 μg 4ºC or below
5-Carboxylcytosine DNA Standard 2 μg 4ºC or below

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
Dot Assay dependent
Not tested in other applications.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with PBS to 50ng/μl. Lyophilized from 10mM Tris-HCl, 0.1mM EDTA.

Preservative

No Preservative

Storage

Store at 4ºC or below. After reconstitution, aliquot immediately and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

5-methylcytosine , 5-hydroxymethylcytosine , 5-formylcytosine , 5-carboxylcytosine

DATA IMAGES

Methylated cytosine DNA standard kit. GTX400004

GTX400004 Dot Image

All five standards are 426 bp with all cytosines either unmodified (lane 1, C), 5-methylcytosine (lane 2, 5mC), 5-hydroxymethylcytosine (lane 3, 5hmC), 5-formylcytosin (lane4, 5fC), 5-carboxycytosine (lane5, 5-caC). DNA marker: 1 kb Plus DNA marker.

Methylated cytosine DNA standard kit. GTX400004

GTX400004 Dot Image

GeneTex methylated cytosine DNA standard kit includes five recombinant double-strand DNA fragments: unmethylated cytosine DNA (C), 5-methylated cytosine DNA (5mC), 5-hydroxymethycytosine cytosine DNA (5hmC), 5-formylcytosine cytosine DNA (5fC), 5-carboxylcytosine cytosine DNA (5caC). Each methylated cytosine DNA was spotted onto a positively charged nylon membrane and subjected to Dot blot assay with corresponding antibodies.
The membrane was incubated with anti-5hmc monoclonal antibody (GTX629701) at a dilution of 1:500 or with anti-5mc monoclonal antibody (GTX629448) at a dilution of 1:500

REFERENCE

Application Reference

Bensberg M et al. Proc Natl Acad Sci U S A 2021; 118 (34) TET2 as a tumor suppressor and therapeutic target in T-cell acute lymphoblastic leukemia.
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Package List Price ($)
$ 299