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Mouse IgG2a isotype control [HOPC-1]

Cat. No. GTX35019

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 HOPC-1

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, in vitro, in vivo
Package
100 μg ($109)
See all Mouse IgG2a isotype control products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
in vitro Assay dependent
in vivo Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Negative control for Mouse IgG2a kappa , Isotype control to Mouse IgG2a kappa , monoclonal Mouse IgG2a isotype control , Mouse IgG2a negative control , Mouse isotype control for IgG2a kappa , Mouse IgG2a kappa isot , Mouse negative control for IgG2a kappa

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

REFERENCE

Application Reference

Vukcevic M et al. J Cell Sci 2013; 126 (Pt 15) : 3485-92 Gain of function in the immune system caused by a ryanodine receptor 1 mutation.
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Package List Price ($)
$ 109