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Mouse IgG2b isotype control [A-1]

Mouse IgG2b isotype control [A-1]. GTX35023

Cat. No. GTX35023

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 A-1

Isotype

 IgG2b

Application

 IHC-P, IHC-Fr, FACS, ELISA, in vitro
Package
100 μg ($79)
See all Mouse IgG2b isotype control products

APPLICATION

Application Note

Use at a concentration/dilution equivalent to that of your experimental primary antibody.

Product Note

This antibody is not suitable for using in any chicken derived sample.

PROPERTIES

Form

Liquid

Buffer

Borate buffered saline

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Mouse isotype control for IgG2c kappa , Isotype control to Mouse IgG2c kappa , Isotype control for Mouse IgG2c kappa , Mouse IgG2c kappa isotype control , Mouse negative control for IgG2c kappa , Mouse IgG2c isotype control , monoclonal Mouse IgG2c isotyp

Background

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding. These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

DATA IMAGES

Mouse IgG2b isotype control [A-1]. GTX35023

GTX35023 ELISA Image

ELISA analysis of serial diluted GTX35023 Mouse IgG2b isotype control [A-1] using mouse immunoglobin antibodies.

REFERENCE

Application Reference

Vukcevic M et al. J Cell Sci 2013; 126 (Pt 15) : 3485-92 Gain of function in the immune system caused by a ryanodine receptor 1 mutation.

Clone Reference

Annunziato F et al. J Exp Med 2002; 196 (3):379-87 Phenotype, localization, and mechanism of suppression of CD4(+)CD25(+) human thymocytes.
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Package List Price ($)
$ 79