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Peroxidase Enhancer

Cat. No. GTX82979

Reference 2

Application

 ICC/IF, IHC
Package
1 kit ($199)
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PRODUCT

Summary

Peroxidase Signal Enhancer

APPLICATION

Application Note

Mixing of reagent: To five ml buffer (reagent B) in a test tube, add one drop of concentrated Enhancing reagent (reagent E) mix well. This ready to use reagent is good for 4-6 hours. Procedure: 1.) After the peroxidase reaction, wash slide with buffer (this buffer should not contain any sodium azide because peroxidase is inactivated) and distilled/ deionized water. 2.) Add few drops of ready to use enhancing reagent for 1-2 minutes. 3.) Add few drops of ready to use AEC reagent, or DAB reagent, follow instructions as outlined for these chromogens.

PROPERTIES

Form

Liquid

Buffer

Reagent B (buffer): 250ml Reagent E (conc. Enhancinig reagent): 3 ml

Preservative

No preservative

Storage

Keep as concentrated solution, aliquot and store at 4ºC. DO NOT FREEZE.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Peroxidase enzyme system is routinely used in Immunohistochemistry (IHC) and Immunocytochemistry (ICC). This Peroxidase Enhancing system increases the level of sensitivity in peroxidase based chromogens (AEC and DAB). This product is used prior to addition of chromogens

REFERENCE

Application Reference

AlSiraj Y et al. J Pharmacol Exp Ther 2021; 377 (3) : 326-335 Therapeutic Assessment of Combination Therapy with a Neprilysin Inhibitor and Angiotensin Type 1 Receptor Antagonist on Angiotensin II-Induced Atherosclerosis, Abdominal Aortic Aneurysms, and Hypertension.
Leng S et al. J Lipid Res 2016; 57 (6) : 1006-16 Ursolic acid enhances macrophage autophagy and attenuates atherogenesis.
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Package List Price ($)
$ 199