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SARS-CoV Spike (SΔ3) antibody [7G12]

Anti-SARS-CoV Spike (SΔ3) antibody [7G12] used in Western Blot and Immunoprecipitation (WB IP). GTX632603
Anti-SARS-CoV Spike (SΔ3) antibody [7G12] used in Western Blot (WB). GTX632603
Anti-SARS-CoV Spike (SΔ3) antibody [7G12] used in Western Blot (WB). GTX632603

Cat. No. GTX632603

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 7G12

Isotype

 IgG1

Application

 WB, IP

Reactivity

 SARS Coronavirus
Package
100 μl ($399),
25 μl ($169)
See all SARS-CoV Spike products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP Assay dependent
Not tested in other applications.

Positive Control

SΔ3-transfected 293T cells

Product Note

This antibody detects SARS-CoV spike protein (S1 subunit), and may have minor cross-reactivity with SARS-CoV-2 spike protein based on data from an external lab. Our internal testing indicates no cross-reactivity with MERS-CoV spike protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

SARS Coronavirus

Immunogen

The immunogen used to generate this antibody corresponds to SARS-CoV SΔ3 (within S1 domain) protein (168-461 a.a.).

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888321

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Human SARS coronavirus Spike protein , Human SARS coronavirus S , SARS-CoV Spike protein , SARS-CoV S

Research Area

DATA IMAGES

Anti-SARS-CoV Spike (SΔ3) antibody [7G12] used in Western Blot and Immunoprecipitation (WB IP). GTX632603

GTX632603 WB IP Image

WB/IP analysis of 293T cells transfected with expression plasmid for wild-type S and mutated forms of S (S(N1056K), S(D1128A), S(N1056K, D1128A)) using GTX632603 SARS-CoV SΔ3 antibody [7G12].

Anti-SARS-CoV Spike (SΔ3) antibody [7G12] used in Western Blot (WB). GTX632603

GTX632603 WB Image

SARS-CoV SΔ3 antibody detects SARS-CoV SΔ3 protein by western blot analysis. Non-transfected (-) and SARS-CoV SΔ3-transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV SΔ3 antibody (GTX632603) diluted at 1:1000.

Anti-SARS-CoV Spike (SΔ3) antibody [7G12] used in Western Blot (WB). GTX632603

GTX632603 WB Image

WB analysis of 293T cells transfected with expression plasmid for wild-type S and mutated forms of S (S(N1056K), S(D1128A), S(N1056K, D1128A)) using GTX632603 SARS-CoV SΔ3 antibody [7G12].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations