PRODUCT
Summary
Tau antibody detects Tau protein, a phosphorylated microtubule-associated protein with a predicted molecular weight of 79 kDa. Tau protein has six isoforms in humans generated from a single gene. Expression changes of these Tau isoforms are controlled during neuronal development and in various pathological conditions. Dysfunction of Tau is associated with neurodegenerative diseases including Alzheimer’s disease and frontotemporal dementia.
APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , SH-SY5Y
Predict Reactivity
Bovine, Dog, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.47 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Tau. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886280
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
microtubule associated protein tau , DDPAC , FTDP-17 , MAPTL , MSTD , MTBT1 , MTBT2 , PPND , PPP1R103 , TAU
Cellular Localization
Cytoplasm , cytosol , Cell membrane; Peripheral membrane protein; Cytoplasmic side , cytoskeleton , Cell projection , axon
Background
This gene encodes the microtubule-associated protein tau (MAPT) whose transcript undergoes complex, regulated alternative splicing, giving rise to several mRNA species. MAPT transcripts are differentially expressed in the nervous system, depending on stage of neuronal maturation and neuron type. MAPT gene mutations have been associated with several neurodegenerative disorders such as Alzheimer's disease, Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX130462 WB Image
Non-transfected (–) and transfected (+) SK-N-SH whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX130462 ICC/IF Image
Tau antibody detects Tau protein at axon by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neurons were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Tau protein stained by Tau antibody (GTX130462) diluted at 1:500.
Red: MAP2, stained by MAP2 antibody [HM-2] (GTX11267) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130462 WB Image
Tau antibody detects Tau protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5 % SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) at a dilution of 1:10000.
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GTX130462 IHC-Fr Image
Tau antibody detects Tau protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse cerebellum.
Green: Tau protein stained by Tau antibody (GTX130462) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130462 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX130462 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX130462 IHC-P Image
Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: Tau stained by Tau antibody (GTX130462) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, a cytoskeleton marker, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130462 WB Image
Untreated (–) and treated (+) SK-N-SH whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Tau antibody (GTX130462) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX130462 IHC-P Image
Tau antibody detects Tau protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Green: Tau stained by Tau antibody (GTX130462) diluted at 1:250.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130462 IHC-Fr Image
Tau antibody detects Tau protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: Tau protein stained by Tau antibody (GTX130462) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
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GTX130462 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membranes were blotted with Tau antibody (GTX130462) diluted at 1:4000 and competitor's antibody (#4019) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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REFERENCE
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