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Trident 10X PBST

Cat. No. GTX30977

Application

 WB, ELISA
Package
100 ml ($59)
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PRODUCT

Summary

Phosphate-Buffered Saline with Tween Solution (10X)

APPLICATION

Application Note

Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8ºC for several weeks. Wash membrane or ELISA plates for 10 minutes each for three times. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.

PROPERTIES

Form

Liquid

Storage

Room temperature. If stored at 2-8ºC, some sodium chloride will be precipitated, if this occurs, bring the buffer to room temperature and mix well till all sodium chloride is dissolved.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

This buffer is supplied as 10X PBS solution containing 1% Tween. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc. This buffer does not contain any sodium azide or mercury preservative and can be used for dilution of all antibodies, including peroxidase. This buffer does not contain any proteins.

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Package List Price ($)
$ 59