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Trident RIPA Lysis Buffer

Trident RIPA Lysis Buffer. GTX400005
Trident RIPA Lysis Buffer. GTX400005

Cat. No. GTX400005

Reference 13

Application

 WB
Package
100 ml ($59)
See all Extraction buffer products
PRODUCT

Summary

Radio-Immunoprecipitation assay lysis buffer
The ingredients of RIPA lysis buffer are as follows:
25 mM Tris pH 7.6
150 mM NaCl
1% Triton X-100
1% sodium deoxycholate
1% SDS

APPLICATION

Application Note

This product contains 1% SDS, which is higher than what is in some other commercial RIPA lysis buffers, and should facilitate a more thorough extraction of nuclear protein. The sample may be viscous following extraction due to unsheared DNA. To decrease this viscosity, we recommend increasing the volume of GTX400005 and trying either sonication or drawing the sample back-and-forth gently (to avoid bubbles) ten times through an 18G blunt needle attached to a 1ml syringe.

Positive Control

A431 , HeLa

PROPERTIES

Form

Liquid

Storage

Store at 2-8ºC.

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

RIPA lysis buffer is used to efficiently lyse most adherent and non-adherent mammalian cells. The extraction of cytosolic, membrane and nuclear proteins can be achieved by using GTX 400005 RIPA Lysis buffer. As the reagent would not adversely affect the antibodies and protein antigens, it often can be used for immunoprecipitation assay.

DATA IMAGES

Trident RIPA Lysis Buffer. GTX400005

GTX400005 WB Image

A431 and HeLa cells were lysed by using GTX400005 RIPA lysis buffer.
Whole cell extracts (30 μg) were assayed by Western Blot analysis.
The membrane was blotted with a cytosolic protein GAPDH antibody (GTX627408), a membrane protein Flotillin1 antibody (GTX104769) and a nuclear protein LaminB2 (GTX628803).
Trident RIPA Lysis Buffer. GTX400005

GTX400005 Image

REFERENCE

Application Reference

Chiu YJ et al. Environ Toxicol 2022; (Epub) Curcumin suppresses cell proliferation and triggers apoptosis in vemurafenib-resistant melanoma cells by downregulating the EGFR signaling pathway.
Ha HA et al. Oncol Rep 2020; (Epub) Novel quinazolinone MJ?33 induces AKT/mTOR?mediated autophagy?associated apoptosis in 5FU?resistant colorectal cancer cells.
Lin LC et al. Int J Med Sci 2020; 17 (14) : 2214-2224 NAD(P)H:quinone oxidoreductase 1 determines radiosensitivity of triple negative breast cancer cells and is controlled by long non-coding RNA NEAT1.
Shen HT et al. Cancers (Basel) 2020; 12 (8) BMI1-Mediated Pemetrexed Resistance in Non-Small Cell Lung Cancer Cells Is Associated with Increased SP1 Activation and Cancer Stemness.
Low HY et al. Cancers (Basel) 2020; 12 (6) Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells.
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Application Reference
Chiu YJ et al. Environ Toxicol 2022; (Epub) Curcumin suppresses cell proliferation and triggers apoptosis in vemurafenib-resistant melanoma cells by downregulating the EGFR signaling pathway.
Ha HA et al. Oncol Rep 2020; (Epub) Novel quinazolinone MJ?33 induces AKT/mTOR?mediated autophagy?associated apoptosis in 5FU?resistant colorectal cancer cells.
Lin LC et al. Int J Med Sci 2020; 17 (14):2214-2224 NAD(P)H:quinone oxidoreductase 1 determines radiosensitivity of triple negative breast cancer cells and is controlled by long non-coding RNA NEAT1.
Shen HT et al. Cancers (Basel) 2020; 12 (8) BMI1-Mediated Pemetrexed Resistance in Non-Small Cell Lung Cancer Cells Is Associated with Increased SP1 Activation and Cancer Stemness.
Low HY et al. Cancers (Basel) 2020; 12 (6) Reciprocal Regulation Between Indoleamine 2,3-Dioxigenase 1 and Notch1 Involved in Radiation Response of Cervical Cancer Stem Cells.
Zhou P et al. Front Cell Infect Microbiol 2019; 9:225
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
Yen CM et al. Cancer Genomics Proteomics 2018; 15 (6):453-460 novel combination of * (as2o3) plus resveratrol in inducing programmed cell death of human neuroblastoma sk-n-sh cells.
Lee CF et al. Biomedicine (Taipei) 2018; 8 (3):15 benzyl isothiocyanate (bitc) triggers mitochondria-mediated apoptotic machinery in human *-resistant oral cancer car cells.
Lu CC et al. In Vivo 2018; 32 (3):677-684 cDNA Microarray Analysis and Influx Transporter OATP1B1 in Liver Cells After Exposure to * Disodium, a Gadolinium-based Contrast Agent in MRI Liver Imaging.
Chen CK et al. Mol Neurobiol 2017; (Epub) TRPV4 Activation Contributes Functional Recovery from Ischemic Stroke via Angiogenesis and Neurogenesis.
Hsu PY et al. J Cell Mol Med 2016; (Epub) MicroRNA let-7g possesses a therapeutic potential for peripheral artery disease.
Tsai JR et al. PLoS One 2015; 10 (12):e0144462 Curcumin Inhibits Non-Small Cell Lung Cancer Cells Metastasis through the Adiponectin/NF-κb/MMPs Signaling Pathway.
Package List Price ($)
$ 59