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YAP1 antibody

KO/KD
Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35195
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35195
Anti-YAP1 antibody used in Western Blot (WB). GTX35195
Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX35195
Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35195
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35195
Anti-YAP1 antibody used in Western Blot (WB). GTX35195
Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35195

Cat. No. GTX35195

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all YAP1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:20 - 1:50
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 155-504 of human YAP1 (NP_001123617.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887749

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yes associated protein 1 , COB1 , YAP , YAP2 , YAP65 , YKI

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2013]

Database

Research Area

DATA IMAGES

Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35195

GTX35195 ICC/IF Image

ICC/IF analysis of C6 cells using GTX35195 YAP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35195

GTX35195 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX35195 YAP1 antibody.
Dilution : 1:100

Anti-YAP1 antibody used in Western Blot (WB). GTX35195

GTX35195 WB Image

WB analysis of various sample lysates using GTX35195 YAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-YAP1 antibody used in Immunoprecipitation (IP). GTX35195

GTX35195 IP Image

IP analysis of HeLa cell lysate using GTX35195 YAP1 antibody.
Antibody amount : 3μg / 200μg lysate
Dilution : 1:1000

Anti-YAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35195

GTX35195 ICC/IF Image

ICC/IF analysis of L929 cells using GTX35195 YAP1 antibody.
Blue : DAPI
Dilution : 1:100

Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35195

GTX35195 IHC-P Image

IHC-P analysis of rat kidney tissue using GTX35195 YAP1 antibody.
Dilution : 1:100

Anti-YAP1 antibody used in Western Blot (WB). GTX35195

GTX35195 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) HeLa cell lysate using GTX35195 YAP1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-YAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35195

GTX35195 IHC-P Image

IHC-P analysis of human placenta tissue using GTX35195 YAP1 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Tao Li et al. bioRxiv 2019; (Epub) Variation in the life history strategy of cells underlies tumor’s functional diversity.
Application : ICC/IF
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399