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Yellow Fever virus NS4B Protein antibody

Anti-Yellow Fever virus NS4B Protein antibody used in Western Blot (WB). GTX134030

Cat. No. GTX134030

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Yellow Fever virus
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.45 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yellow Fever virus

Immunogen

Recombinant protein encompassing a sequence within the center region of Yellow Fever Virus NS4B Protein (Yellow fever virus (strain 17D vaccine)). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887179

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Yellow Fever Virus Non-structural protein 4B , Yellow Fever Virus NS4B , YFV Non-structural protein 4B , YFV NS4B

Research Area

DATA IMAGES

Anti-Yellow Fever virus NS4B Protein antibody used in Western Blot (WB). GTX134030

GTX134030 WB Image

Yellow Fever viral lysate (0.1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Yellow Fever virus NS4B Protein antibody (GTX134030) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Yellow Fever virus NS4B Protein antibody Cat. No. GTX134030

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 15-Feb-2024
Score :
Application Tested : ICC/IF
Sample : VeroE6 cells with/without ZIKV virus infection (MOI 0.1, 24hr)
Blocking : PBS, 2% BSA, 0.5Hr
Fixation : 4 % formal- dehyde
Permeabilization /Antigen retrieval : PBS, 0.5 % Triton X-100, 20 mM glycine
Primary Antibody : 1:1000, °C, Hr
Figure Note :
Orange : Primary antibody
Blue : DAPI
Images were acquired using a Leica SP8 Laser Scanning Confocal Microscope with a 63x oil objective (Leica) and Leica Application Suit X software.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169