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iNOS antibody

Orthogonal
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX17504
Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX17504
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17504
Anti-iNOS antibody used in Western Blot (WB). GTX17504
Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17504

Cat. No. GTX17504

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Fr, IHC, IM

Reactivity

 Human, Mouse, Rat, Rabbit, Cat, Pig, Amphibians, Turbot, Avian
Package
200 μl ($649)
See all iNOS products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:200 - 1:800
ICC/IF 1:50
IHC-P 1:20
IHC-Fr Assay dependent
IHC Assay dependent
IM Assay dependent
Not tested in other applications.

Calculated MW

131 kDa. ( Note )

Product Note

This antibody does not detect other NOS isoforms.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.1% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to residues D(17) L K E E K D I N N N V K K T(31) of mouse iNOS.

Purification

Ammonium sulfate precipitation

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

nitric oxide synthase 2, inducible , MAC-NOS , NOS-II , Nos-2 , Nos2a , i-NOS , iNOS

Cellular Localization

Cytoplasm, cytosol

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]

Database

Research Area

DATA IMAGES

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX17504

GTX17504 IHC-P Image

IHC-P analysis of human heart tissue using GTX17504 iNOS antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-iNOS antibody used in IHC (Paraffin sections) (IHC-P). GTX17504

GTX17504 IHC-P Image

IHC-P analysis of human lung tissue using GTX17504 iNOS antibody.
Left : Primary antibody
Right : Negative control without primary antibody
Antigen retrieval : heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes
Dilution : 1:200

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17504

GTX17504 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX17504 iNOS antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

Anti-iNOS antibody used in Western Blot (WB). GTX17504

GTX17504 WB Image

Orthogonal

WB analysis of 20 ug of RAW264 whole cell lysate untreated (left lane) or stimulated with LPS at 1 μg/mL for 16 hours (right lane) using GTX17504 iNOS antibody.
Dilution : 1:1000

Anti-iNOS antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX17504

GTX17504 ICC/IF Image

ICC/IF analysis of A549 cells using GTX17504 iNOS antibody. Cells were probed without (right) or with(left) an antibody.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : formaldehyde
Dilution : 1:20 overnight at 4ºC

REFERENCE

Application Reference

Sun Y et al. Immun Inflamm Dis 2022; 10 (7) : e641 TRPM7 promotes lipopolysaccharide-induced inflammatory dysfunction in renal tubular epithelial cells.
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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 649