APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:1000 |
| 1:250 |
| Assay dependent |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.1% BSA
Preservative
0.05% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
Batch dependent (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Synthetic peptide corresponding to residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein.
Purification
Protein G purified
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1
Cellular Localization
Nucleus,Cytoplasmic vesicle
Background
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]
Database
Research Area
DATA IMAGES
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/templates/default/js/lightbox/images/loading.gif "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
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GTX15666 ICC/IF Image
ICC/IF analysis of 293 untreated cells (left panel) or stimulated cells with 100 mJ UV (right panel) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
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GTX15666 WB Image
WB analysis of 40 ug of lysate from HeLa cells untreated (Lane 1), treated with 10uM Camptothecin for 4h (Lane 2) or 12h (Lane 3) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Dilution : 1:1000
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GTX15666 ICC/IF Image
ICC/IF analysis of HT-29 cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Left : Primary antibody
Right : Negative control without primary antibody
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes
Dilution : 1:50 and incubated overnight
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GTX15666 ICC/IF Image
ICC/IF analysis of HeLa cells irradiated with UV for 4 hours using GTX15666 ATM (phospho Ser1981) antibody [10H11]. Panel e is a no primary antibody control.
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : 4% paraformaldehyde
Permeabilization : 0.1% Trito X-100 for 10 minutes
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GTX15666 ICC/IF Image
ICC/IF analysis of HeLa cells left untreated (left panels) or treated with 10uM Camptothecin for 4 hours (right panels) using GTX15666 ATM (phospho Ser1981) antibody [10H11].
Green : Primary antibody
Blue : Nuclei
Red : Actin
Fixation : Formalin
Permeabilization : 0.1% Triton X-100 in TBS for 10 minutes
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GTX15666 WB Image
WB analysis of gamma-irradiated HeLa cells using GTX15666 ATM (phospho Ser1981) antibody [10H11].
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REFERENCE
There are currently no references for ATM (phospho Ser1981) antibody [10H11] (GTX15666). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for ATM (phospho Ser1981) antibody [10H11] (GTX15666). Be the first to share your experience with this product.
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/small/GTX15666/GTX15666_280_ICC-IF_w_23060620_691.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666](/upload/website/prouct_img/small/GTX15666/GTX15666_1510_WB_w_23060620_543.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/small/GTX15666/GTX15666_1998_ICC-IF_w_23060620_886.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/small/GTX15666/GTX15666_278_ICC-IF_w_23060620_686.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/small/GTX15666/GTX15666_279_ICC-IF_w_23060620_514.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666](/upload/website/prouct_img/small/GTX15666/GTX15666_1511_WB_w_23060620_125.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/normal/GTX15666/GTX15666_280_ICC-IF_w_23060620_691.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666](/upload/website/prouct_img/normal/GTX15666/GTX15666_1510_WB_w_23060620_543.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/normal/GTX15666/GTX15666_1998_ICC-IF_w_23060620_886.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/normal/GTX15666/GTX15666_278_ICC-IF_w_23060620_686.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666](/upload/website/prouct_img/normal/GTX15666/GTX15666_279_ICC-IF_w_23060620_514.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX15666")
![Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666](/upload/website/prouct_img/normal/GTX15666/GTX15666_1511_WB_w_23060620_125.webp "Anti-ATM (phospho Ser1981) antibody [10H11] used in Western Blot (WB). GTX15666")