AUF1 antibody [N1C1]

Name: AUF1 antibody [N1C1]
Brand: GeneTex
SKU: GTX101813
Price: 399.00 USD
Rating: 3 (1 reviews)

Anti-AUF1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101813

Anti-AUF1 antibody [N1C1] used in Western Blot (WB). GTX101813

Cat. No. GTX101813

Reference 1

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($399), 25 μl ($169)

See all AUF1 products

View product citations for antibody GTX101813 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A431

Predict Reactivity

Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human AUF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11166847

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

heterogeneous nuclear ribonucleoprotein D , AUF1 , AUF1A , HNRPD , P37 , hnRNPD0

Cellular Localization

Nucleus

Background

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq]

Database

Gene ID: 3184 HNRNPD
UniProt: Q14103 HNRNPD

Research Area

DATA IMAGES

GTX101813 ICC/IF Image

AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AUF1 protein stained by AUF1 antibody [N1C1] (GTX101813) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

GTX101813 ICC/IF Image

AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AUF1 protein stained by AUF1 antibody [N1C1] (GTX101813) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

GTX101813 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX101813 diluted at 1:1000

REFERENCE

Application Reference

Chang JF et al. Toxicol Sci 2014; (Epub) nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.

Application : WB

Reactivity : Human

Submit a Reference

REVIEW