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C9orf72 antibody [GT779]

KO/KD Overexpression
Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041
Anti-C9orf72 antibody [GT779] used in Immunoprecipitation (IP). GTX632041
Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Anti-C9orf72 antibody [GT779] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632041

Cat. No. GTX632041

Reference 14
2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT779

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all C9orf72 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

mouse brain , rat brain

Product Note

Knockdown/Knockout validation was supported by customer review data

Predict Reactivity

Bovine, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human C9orf72. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2784546

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C9orf72-SMCR8 complex subunit , ALSFTD , DENNL72 , FTDALS , FTDALS1

Database

Research Area

DATA IMAGES

Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041

GTX632041 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT779] used in Immunoprecipitation (IP). GTX632041

GTX632041 IP Image

Immunoprecipitation of C9orf72 protein from SK-N-SH whole cell extracts using 5 μg of C9orf72 antibody [GT779] (GTX632041).
Western blot analysis was performed using C9orf72 antibody [GT779] (GTX632041).
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041

GTX632041 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041

GTX632041 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm in human colon cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human colon cancer.
C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041

GTX632041 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with C9orf72 antibody [GT779] (GTX632041) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041

GTX632041 IHC-P Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse hippocampus.
C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-C9orf72 antibody [GT779] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632041

GTX632041 ICC/IF Image

C9orf72 antibody [GT779] detects C9orf72 protein at cytoplasm by immunofluorescent analysis.
Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: C9orf72 stained by C9orf72 antibody [GT779] (GTX632041) diluted at 1:500.
Red: Tau, a Tau marker, stained by Tau antibody (GTX130462) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

F Limone et al. Sci Transl Med 2024; 16 (732) : eadg7895 Myeloid and lymphoid expression of C9orf72 regulates IL-17A signaling in mice
SE Salomonsson et al. Sci Rep 2024; 14 (1) : 828 Validated assays for the quantification of C9orf72 human pathology
Application : WB
Reactivity : Human
He L et al. Cell Death Differ 2023; 30 (3) : 716-730 C9orf72 functions in the nucleus to regulate DNA damage repair.
ZS Chen et al. Nat Commun 2023; 14 (1) : 8420 Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD
Application : WB
J?lg J et al. EMBO Rep 2023; 24 (8) : e55895 C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains.
Show More +
Submit a Reference

REVIEW

C9orf72 antibody [GT779] Cat. No. GTX632041

Rating
( Average 4 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 3 based on 1 users reviews)
Anti-C9orf72 antibody [GT779] used in IHC (Paraffin sections) (IHC-P). GTX632041
Date : Anonymous submitted on 20-Oct-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : Mouse brain tissue section, hippocampus, CA2-3 region
Blocking : Calf serum, 30°C, 1Hr
Fixation : PFA
Permeabilization /Antigen retrieval : TBS with 0.3% tritonX / pH9 antigen retireval buffer
Primary Antibody : 1:1000, 16°C, 4Hr
Anti-C9orf72 antibody [GT779] used in Western Blot (WB). GTX632041
Date : Anonymous submitted on 18-Jan-2017
Score :
Application Tested : WB
Sample Species : Ms
Sample : Macrophages
Primary Antibody : 1:500, 4°C, 16Hr
Lane Description : Lane 1: wild type, Lane 2: heterozygous C9orf72 disruption, Lane 3: homozygous C9orf72 disruption
Submit a Review
Application Reference
F Limone et al. Sci Transl Med 2024; 16 (732):eadg7895 Myeloid and lymphoid expression of C9orf72 regulates IL-17A signaling in mice
SE Salomonsson et al. Sci Rep 2024; 14 (1):828 Validated assays for the quantification of C9orf72 human pathology
Application : WB
Reactivity : Human
He L et al. Cell Death Differ 2023; 30 (3):716-730 C9orf72 functions in the nucleus to regulate DNA damage repair.
ZS Chen et al. Nat Commun 2023; 14 (1):8420 Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD
Application : WB
Seidel M et al. Curr Res Neurobiol 2023; 5:100105 Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models.
J?lg J et al. EMBO Rep 2023; 24 (8):e55895 C9orf72 protein quality control by UBR5-mediated heterotypic ubiquitin chains.
Mira Seidel et al. Curr Opin Neurobiol 2023; 5 (2023):100105 Propranolol reduces the accumulation of cytotoxic aggregates in C9orf72-ALS/FTD in vitro models
Application : WB
Reactivity : Human
Ghaffari LT et al. iScience 2023; 26 (6):106959 Differential response of C9orf72 transcripts following neuronal depolarization.
Pang W et al. iScience 2023; 26 (5):106579 C9ORF72 suppresses JAK-STAT mediated inflammation.
Liu Y et al. Mol Cell Neurosci 2022; 121:103756 Hyperactivity of Purkinje cell and motor deficits in C9orf72 knockout mice.
Bauer CS et al. Acta Neuropathol 2022; 144 (3):437-464 An interaction between synapsin and C9orf72 regulates excitatory synapses and is impaired in ALS/FTD.
Liu Y et al. Nat Commun. 2021; 12 (1):847 Variant-selective stereopure oligonucleotides protect against pathologies associated with C9orf72-repeat expansion in preclinical models.
Application : WB
Reactivity : Human
Laflamme C et al. Elife 2019; 8 Implementation of an antibody characterization procedure and application to the major ALS/FTD disease gene C9ORF72.
Application : WB, ICC/IF, IP
Reactivity : Human
Carl Laflamme et al. bioRxiv 2018; (Epub) Implementation of an antibody characterization process: Application to the major ALS/FTD disease gene C9ORF72.
Application : WB, ICC/IF, IP
Reactivity : Human, Mouse
SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169