CCR4 antibody [HL2481]

Cat. No. GTX638830

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL2481
Isotype	IgG
Application	WB, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

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Datasheet

View product citations for antibody GTX638830 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:500-1:3000
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

41 kDa. ( Note )

Product Note

This antibody was raised against human CCR4 Intracellular domain.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human CCR4. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

C-C motif chemokine receptor 4 , CC-CKR-4 , CD194 , CKR4 , CMKBR4 , ChemR13 , HGCN:14099 , K5-5

Cellular Localization

Cell membrane; Multi-pass membrane protein

Background

The protein encoded by this gene belongs to the G-protein-coupled receptor family . It is a receptor for the CC chemokine - MIP-1, RANTES, TARC and MCP-1. Chemokines are a group of small polypeptide, structurally related molecules that regulate cell trafficking of various types of leukocytes. The chemokines also play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. [provided by RefSeq, Jul 2008]

Database

Gene ID: 1233 CCR4
UniProt: P51679 CCR4

Research Area

DATA IMAGES

GTX638830 WB Image

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

GTX638830 WB Image

Non-transfected (–) and transfected (+) bolied and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638830 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX638830 WB Image

Unboiled SW480 whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (WCE: whole cell extract; ME: membrane extract)

GTX638830 IHC-P Image

CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
CCR4 stained by CCR4 antibody [HL2481] (GTX638830) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638830 IHC-P Image

CCR4 antibody [HL2481] detects CCR4 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded rat thymus gland.
CCR4 stained by CCR4 antibody [HL2481] (GTX638830) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX638830 WB Image

Non-transfected (–) and transfected (+) boiled and unboiled SW480 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with CCR4 antibody [HL2481] (GTX638830) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

CCR4 antibody [HL2481]

Cat. No. GTX638830

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Product Note

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX638830 WB Image

GTX638830 WB Image

GTX638830 WB Image

GTX638830 WB Image

GTX638830 IHC-P Image

GTX638830 IHC-P Image

GTX638830 WB Image

REFERENCE

REVIEW

Product

Research

Support

Company

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