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Claudin 1 antibody

Comparison Orthogonal
Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842
Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842
Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842
Anti-Claudin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134842
Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842
Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842

Cat. No. GTX134842

Reference 5

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Claudin 1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

23 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.43 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide encompassing a sequence within the Intracellular domain of human Claudin 1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887357

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

claudin 1 , CLD1 , ILVASC , SEMP1

Cellular Localization

Cell membrane

Background

Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842

GTX134842 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membranes were blotted with Claudin 1 antibody (GTX134842) diluted at 1:500 and competitor's antibody diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842

GTX134842 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 1 antibody (GTX134842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842

GTX134842 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 1 antibody (GTX134842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Claudin 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX134842

GTX134842 ICC/IF Image

Claudin 1 antibody detects Claudin 1 protein at cell membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Claudin 1 stained by Claudin 1 antibody (GTX134842) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842

GTX134842 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 1 antibody (GTX134842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Claudin 1 antibody used in Western Blot (WB). GTX134842

GTX134842 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Claudin 1 antibody (GTX134842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Tsou HH et al. Toxicology 2022; 479 : 153318 Effect of heated tobacco products and traditional cigarettes on pulmonary toxicity and SARS-CoV-2-induced lung injury.
Walter Muranyi et al. iScience 2022; 25 (6) : 104383 Immortalized human choroid plexus endothelial cells enable an advanced endothelial-epithelial two-cell type in?vitro model of the choroid plexus.
Meng-Hui Zhang et al. Bioengineered 2022; 13 (4) : 10047-10060 Cleavage stimulation factor 2 promotes malignant progression of liver hepatocellular carcinoma by activating phosphatidylinositol 3'-kinase/protein kinase B/mammalian target of rapamycin pathway.
Application : WB
Arciniega-Mart?nez IM et al. Mol Med Rep 2022; 25 (2) CD4+/IL?4+ lymphocytes of the lamina propria and substance P promote colonic protection during acute stress.
Application : WB
Reactivity : Mouse
Terasaki M et al. Cancer Genomics Proteomics 2021; 18 (2) : 133-146 A Fucoxanthinol Induces Apoptosis in a Pancreatic Intraepithelial Neoplasia Cell.
Application : WB
Reactivity : Mouse
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169