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DLK1 antibody [3A10]

Anti-DLK1 antibody [3A10] used in ELISA (ELISA). GTX60511
Anti-DLK1 antibody [3A10] used in Flow cytometry (FACS). GTX60511
Anti-DLK1 antibody [3A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60511
Anti-DLK1 antibody [3A10] used in Western Blot (WB). GTX60511

Cat. No. GTX60511

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3A10

Isotype

 IgG1

Application

 WB, ICC/IF, FACS, ELISA, PLA

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all DLK1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
PLA Assay dependent
Not tested in other applications.

Calculated MW

41 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human DLK1 expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

delta like non-canonical Notch ligand 1 , DLK , DLK-1 , Delta1 , FA1 , PREF1 , Pref-1 , ZOG , pG2

Cellular Localization

Membrane; Single-pass type I membrane protein,Cytoplasm

Background

This gene encodes a transmembrane protein containing six epidermal growth factor repeats. The protein is involved in the differentiation of several cell types, including adipocytes; it is also thought to be a tumor suppressor. It is one of several imprinted genes located in a region of on chr 14q32. Certain mutations in this imprinted region can cause phenotypes similar to maternal and paternal uniparental disomy of chromosome 14 (UPD14). This gene is expressed from the paternal allele. A polymorphism within this gene has been associated with child and adolescent obesity. The mode of inheritance for this polymorphism is polar overdominance; this non-Mendelian inheritance pattern was first described in sheep with the callipyge phenotype, which is characterized by muscle hypertrophy and decreased fat mass. [provided by RefSeq, Mar 2010]

Database

Research Area

DATA IMAGES

Anti-DLK1 antibody [3A10] used in ELISA (ELISA). GTX60511

GTX60511 ELISA Image

ELISA analysis of antigen using GTX60511 DLK1 antibody [3A10].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

Anti-DLK1 antibody [3A10] used in Flow cytometry (FACS). GTX60511

GTX60511 FACS Image

FACS analysis of NIH3T3 cells using GTX60511 DLK1 antibody [3A10].
Green : DLK1
Red : negative control

Anti-DLK1 antibody [3A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60511

GTX60511 ICC/IF Image

ICC/IF analysis of U251 cells using GTX60511 DLK1 antibody [3A10].
Green : DLK1
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-DLK1 antibody [3A10] used in Western Blot (WB). GTX60511

GTX60511 WB Image

WB analysis of human DLK1 (AA: 174-349) recombinant protein using GTX60511 DLK1 antibody [3A10].

REFERENCE

Application Reference

Jung S et al. Nat Commun 2024; 15 (1) : 158 Role of UPF1-LIN28A interaction during early differentiation of pluripotent stem cells.
JK Karapurkar et al. J Exp Clin Cancer Res 2023; 42 (1) : 121 CRISPR/Cas9-based genome-wide screening of the deubiquitinase subfamily identifies USP3 as a protein stabilizer of REST blocking neuronal differentiation and promotes neuroblastoma tumorigenesis
Application : PLA
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399