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Dengue virus Envelope protein antibody [HL2089]

Recombinant antibody
Anti-Dengue virus Envelope protein antibody [HL2089] used in Western Blot (WB). GTX637997
Anti-Dengue virus Envelope protein antibody [HL2089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637997
Anti-Dengue virus Envelope protein antibody [HL2089] used in Western Blot (WB). GTX637997

Cat. No. GTX637997

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2089

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
ICC/IF Assay dependent
Not tested in other applications.

Product Note

The validated Dengue virus serotype for each application may differ. Please refer to the validated data. This antibody does not cross-react with YFV, JEV, Zika, and CHIKV Envelope protein.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Recombinant fragment of Dengue virus Envelope protein. (strain Thailand/16681/1984)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Dengue virus Envelope protein antibody [HL2089] used in Western Blot (WB). GTX637997

GTX637997 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Type 2 Envelope protein antibody [HL2089] (GTX637997) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus Envelope protein antibody [HL2089] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637997

GTX637997 ICC/IF Image

Immunofluorescent analysis of Dengue virus infected cells using Dengue virus Envelope protein antibody [HL2089] antibody (GTX637997).
Sample: Multiple virus infected cells slide.
Green: Dengue virus Envelope protein antibody [HL2089] antibody (GTX637997) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Dengue virus Envelope protein antibody [HL2089] used in Western Blot (WB). GTX637997

GTX637997 WB Image

Non-infected (–) and infected (+) BHK-21 whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with Dengue virus Envelope protein antibody [HL2089] (GTX637997) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

Dengue virus Envelope protein antibody [HL2089] Cat. No. GTX637997

Rating ( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 13-Nov-2023
Score :
Application Tested : ICC/IF
Sample Species : DENV2
Sample : Huh-7 cells with/without Dengue virus 2 infection
Primary Antibody : 1:1000, °C, Hr
Package List Price ($)
$ 399
$ 169
Custom Conjugations