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Dengue virus NS3 protein antibody [HL2239]

Recombinant antibody
Anti-Dengue virus NS3 protein antibody [HL2239] used in Western Blot (WB). GTX638284
Anti-Dengue virus NS3 protein antibody [HL2239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638284
Anti-Dengue virus NS3 protein antibody [HL2239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638284

Cat. No. GTX638284

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL2239

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Dengue virus 1, Dengue virus 2, Dengue virus 3, Dengue virus 4
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:200000
ICC/IF Assay dependent
Not tested in other applications.

Product Note

The validated Dengue virus serotype for each application may differ. Please refer to the validated data.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Dengue virus

Immunogen

Recombinant fragment of Dengue virus NS3 protein. (strain Thailand/16681/1964)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

DATA IMAGES

Anti-Dengue virus NS3 protein antibody [HL2239] used in Western Blot (WB). GTX638284

GTX638284 WB Image

Non-infected and infected BHK-21 whole cell extracts (5 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Dengue virus NS3 protein antibody [HL2239] (GTX638284) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Dengue virus NS3 protein antibody [HL2239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638284

GTX638284 ICC/IF Image

Dengue virus NS3 protein antibody [HL2239] detects Dengue virus NS3 protein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min.
Green: Dengue virus NS3 protein stained by Dengue virus NS3 protein antibody [HL2239] (GTX638284) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Dengue virus NS3 protein antibody [HL2239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638284

GTX638284 ICC/IF Image

Immunofluorescent analysis of Dengue virus infected cells using Dengue virus NS3 protein antibody [HL2239] antibody (GTX638284).
Sample: Multiple virus infected cells slide.
Green: Dengue virus NS3 protein antibody [HL2239] antibody (GTX638284) diluted at 1:100.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations