APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS, 0.05% BSA, 50% Glycerol
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.2 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
A synthesized peptide derived from human eIF4A3.
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
eukaryotic translation initiation factor 4A3 , DDX48 , Fal1 , MUK34 , NMP265 , NUK34 , RCPS , eIF-4A-III , eIF4A-III , eIF4AIII
Cellular Localization
Nucleus , Nucleus speckle , Cytoplasm
Background
This gene encodes a member of the DEAD box protein family. DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a nuclear matrix protein. Its amino acid sequence is highly similar to the amino acid sequences of the translation initiation factors eIF4AI and eIF4AII, two other members of the DEAD box protein family. [provided by RefSeq, Jul 2008]
Database
Research Area
DATA IMAGES
![Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243](/templates/default/js/lightbox/images/loading.gif "Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243")
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GTX03243 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF4A3 antibody [GT1331] (GTX03243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX03243 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with EIF4A3 antibody [GT1331] (GTX03243) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX03243 WB Image
WB analysis of various samples using GTX03243 EIF4A3 antibody [GT1331].
Dilution : 1:1000
Loading : 25μg per lane
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GTX03243 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX03243 EIF4A3 antibody [GT1331].
Blue : DAPI for nuclear staining
Dilution : 1:100
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REFERENCE
There are currently no references for EIF4A3 antibody [GT1331] (GTX03243). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for EIF4A3 antibody [GT1331] (GTX03243). Be the first to share your experience with this product.
![Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243](/upload/website/prouct_img/small/GTX03243/GTX03243_4000001373_20210709_WB_w_23053123_465.webp "Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243")
![Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243](/upload/website/prouct_img/small/GTX03243/GTX03243_4000001373_20210709_WB_2_w_23053123_724.webp "Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243")
![Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243](/upload/website/prouct_img/small/GTX03243/GTX03243_49_WB_w_23053123_493.webp "Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243")
![Anti-EIF4A3 antibody [GT1331] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03243](/upload/website/prouct_img/small/GTX03243/GTX03243_20210615_ICCIF_48_w_23053123_720.webp "Anti-EIF4A3 antibody [GT1331] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03243")
![Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243](/upload/website/prouct_img/normal/GTX03243/GTX03243_4000001373_20210709_WB_w_23053123_465.webp "Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243")
![Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243](/upload/website/prouct_img/normal/GTX03243/GTX03243_4000001373_20210709_WB_2_w_23053123_724.webp "Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243")
![Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243](/upload/website/prouct_img/normal/GTX03243/GTX03243_49_WB_w_23053123_493.webp "Anti-EIF4A3 antibody [GT1331] used in Western Blot (WB). GTX03243")
![Anti-EIF4A3 antibody [GT1331] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03243](/upload/website/prouct_img/normal/GTX03243/GTX03243_20210615_ICCIF_48_w_23053123_720.webp "Anti-EIF4A3 antibody [GT1331] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03243")