APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:1000-1:10000 |
Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human GNAT1 protein, and it does not cross react with human GNAI1, GNAI2, GNAI3, GNAT2, GNAZ, and GNAO1 protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human GNAT1
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
G protein subunit alpha transducin 1 , CSNB1G , CSNBAD3 , GBT1 , GNATR
Cellular Localization
Membrane
Background
Transducin is a 3-subunit guanine nucleotide-binding protein (G protein) which stimulates the coupling of rhodopsin and cGMP-phoshodiesterase during visual impulses. The transducin alpha subunits in rods and cones are encoded by separate genes. This gene encodes the alpha subunit in rods. This gene is also expressed in other cells, and has been implicated in bitter taste transduction in rat taste cells. Mutations in this gene result in autosomal dominant congenital stationary night blindness. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Feb 2009]
Database
Research Area
DATA IMAGES
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GTX638098 WB Image
Human tissue extract (5 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638098 IHC-P Image
GNAT1 antibody [HL2126] detects GNAT1 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat eye.Green: GNAT1 stained by GNAT1 antibody [HL2126] (GTX638098) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638098 IHC-P Image
GNAT1 antibody [HL2126] detects GNAT1 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse eye.Green: GNAT1 stained by GNAT1 antibody [HL2126] (GTX638098) diluted at 1:100.Red: beta Tubulin 3/ Tuj1 stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638098 WB Image
Non-transfected and transfected 293T whole cell extracts were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638098 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638098 WB Image
Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638098 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GNAT1 antibody [HL2126] (GTX638098) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
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REVIEW
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