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GSK3 beta antibody [3D10]

Anti-GSK3 beta antibody [3D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83315
Anti-GSK3 beta antibody [3D10] used in Flow cytometry (FACS). GTX83315
Anti-GSK3 beta antibody [3D10] used in Western Blot (WB). GTX83315
Anti-GSK3 beta antibody [3D10] used in IHC (Paraffin sections) (IHC-P). GTX83315

Cat. No. GTX83315

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3D10

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

 Human, Mouse, Rat, Monkey
Package
100 μl ($399)
See all GSK3 beta products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

47 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human GSK3B expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_11171858

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase, belonging to the glycogen synthase kinase subfamily. It is involved in energy metabolism, neuronal cell development, and body pattern formation. Polymorphisms in this gene have been implicated in modifying risk of Parkinson disease, and studies in mice show that overexpression of this gene may be relevant to the pathogenesis of Alzheimer disease. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Sep 2009]

Database

Research Area

DATA IMAGES

Anti-GSK3 beta antibody [3D10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX83315

GTX83315 ICC/IF Image

ICC/IF analysis of NIH3T3 (left) and U251 (right) cells using GTX83315 GSK3 beta antibody [3D10].
Green : GSK3 beta
Blue: DRAQ5 fluorescent DNA dye
Red: Actin filaments

Anti-GSK3 beta antibody [3D10] used in Flow cytometry (FACS). GTX83315

GTX83315 FACS Image

FACS analysis of HeLa cells using GTX83315 GSK3 beta antibody [3D10].
Green : GSK3 beta
Purple : negative control

Anti-GSK3 beta antibody [3D10] used in Western Blot (WB). GTX83315

GTX83315 WB Image

WB analysis of A549 (1), K562 (2), PC-12 (3), NIH3T3 (4), and HEK293 (5) cell lysate using GTX83315 GSK3 beta antibody [3D10].

Anti-GSK3 beta antibody [3D10] used in IHC (Paraffin sections) (IHC-P). GTX83315

GTX83315 IHC-P Image

IHC-P analysis of human lung cancer (left) and breast cancer tissue (right) using GTX83315 GSK3 beta antibody [3D10].

REFERENCE

Application Reference

Chien T et al. Mol Psychiatry 2018; (Epub) GSK3β negatively regulates TRAX, a scaffold protein implicated in mental disorders, for NHEJ-mediated DNA repair in neurons.
Application : WB
Reactivity : Rat
Tang W et al. Int J Clin Exp Med 2015; 8 (4) : 5897-903 GSK3β expression and phosphorylation during neuronal maturation in the rat dorsal root ganglion.
Application : ICC/IF
Reactivity : Rat
Submit a Reference

REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399