GSK3 beta antibody [GT1619]

Cat. No. GTX635886

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1619
Isotype	IgG2b
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse, Rat
Package	100 μl ($399), 25 μl ($169)

See all GSK3 beta products

Datasheet

View product citations for antibody GTX635886 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

47 kDa. ( Note )

Predict Reactivity

Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human GSK3 beta. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2909915

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

glycogen synthase kinase 3 beta

Cellular Localization

Cytoplasm,Nucleus,Cell membrane

Background

The protein encoded by this gene is a serine-threonine kinase belonging to the glycogen synthase kinase subfamily. It is a negative regulator of glucose homeostasis and is involved in energy metabolism, inflammation, ER-stress, mitochondrial dysfunction, and apoptotic pathways. Defects in this gene have been associated with Parkinson disease and Alzheimer disease. [provided by RefSeq, Aug 2017]

Database

Gene ID: 2932 GSK3B
UniProt: P49841 GSK3B

Research Area

DATA IMAGES

GTX635886 WB Image

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT1619] (GTX635886) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX635886 ICC/IF Image

GSK3 beta antibody detects GSK3 beta protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: GSK3 beta stained by GSK3 beta antibody (GTX635886) diluted at 1:500.

GTX635886 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT1619] (GTX635886) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX635886 IHC-P Image

GSK3 beta antibody [GT1619] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GSK3 beta stained by GSK3 beta antibody [GT1619] (GTX635886) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635886 WB Image

Wild-type (WT) and GSK3B knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [GT1619] (GTX635886) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX635886 IHC-P Image

GSK3 beta antibody [GT1619] detects GSK3 beta protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GSK3 beta stained by GSK3 beta antibody [GT1619] (GTX635886) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635886 IHC-P Image

GSK3 beta antibody [GT1619] detects GSK3 beta protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
GSK3 beta stained by GSK3 beta antibody [GT1619] (GTX635886) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635886 IHC-P Image

GSK3 beta antibody [GT1619] detects GSK3 beta protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
GSK3 beta stained by GSK3 beta antibody [GT1619] (GTX635886) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

GSK3 beta antibody [GT1619]

Cat. No. GTX635886

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

Predict Reactivity

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX635886 WB Image

GTX635886 ICC/IF Image

GTX635886 WB Image

GTX635886 IHC-P Image

GTX635886 WB Image

GTX635886 IHC-P Image

GTX635886 IHC-P Image

GTX635886 IHC-P Image

REFERENCE

REVIEW

Product

Research

Support

Company

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