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Human G-CSF protein (active)

Comparison
Human G-CSF protein (active). GTX138166-pro
Human G-CSF protein (active). GTX138166-pro

Cat. No. GTX138166-pro

Application

 Functional Assay

Species

 Human
Package
100 μg ($799)
See all Human G-CSF protein products
PRODUCT

Summary

G-CSF (Granulocyte-colony stimulating factor/CSF3) is the major growth factor driving the generation of mature granulocytes from committed bone marrow myeloid progenitors. This biologically active recombinant human G-CSF is an 18.7 kDa globular protein formed by a 174-amino acid polypeptide with two intramolecular disulfide bonds. Human and murine G-CSFs are highly similar and show reciprocal biological cross-reactivity.

APPLICATION

Application Note

The ED₅₀ was determined by a cell proliferation assay using murine NFS-60 cells is ≤ 0.1 ng/ml, corresponding to a specific activity of ≥ 1 x 10⁷ units/mg.

Observed MW

15 kDa.

PROPERTIES

Form

Lyophilized powder

Buffer

Reconstitute with ddH₂O to 0.1 mg/ml. Lyophilized from 50mM PBS (pH7.4), 200mM NaCl, 5% Trehalose.

Preservative

No preservative

Storage

Store at 4ºC or below. After reconstitution, keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. For long-term storage after reconstitution, aliquot and store at -70ºC or below. Do not vortex.

Region/Sequence

Human full-length G-CSF protein (31-204 a.a. of NP_757373.1, signal peptide removed)

Expression System

E. Coli

Purity

>98%

Endotoxin

< 1 EU/ug

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

colony stimulating factor 3 , C17orf33 , CSF3OS , GCSF

Cellular Localization

Secreted

Background

The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Alternatively spliced transcript variants have been described for this gene. [provided by RefSeq, May 2010]

Database

Research Area

DATA IMAGES

Human G-CSF protein (active). GTX138166-pro

GTX138166-pro Functional Assay Image

Comparison

Serial dilutions of Human G-CSF protein (active) were added to murine NFS-60 cells. Cell proliferation was measured and the linear portion of the curve was used to calculate the ED50.

Human G-CSF protein (active). GTX138166-pro

GTX138166-pro Image

1 μg of GTX138166-pro Human G-CSF protein (active) was analyzed using SDS-PAGE and stained with coomassie blue and captured by monochrome camera.

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REVIEW

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Package List Price ($)
$ 799