APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
Assay dependent |
Assay dependent |
1:100-1:1000 |
Not tested in other applications.
Calculated MW
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human IP3 Receptor I. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
inositol 1,4,5-trisphosphate receptor 1 , D6Pas2 , Gm10429 , IP3R 1 , IP3R1 , InsP3R , Ip3r , Itpr-1 , P400 , Pcp-1 , Pcp1 , insP3R1 , opt , wblo
Cellular Localization
Endoplasmic reticulum membrane
Database
Research Area
DATA IMAGES
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GTX637015 IHC-P Image
IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
The expression pattern is based on the publication:PMID: 25409824
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GTX637015 WB Image
Whole cell extract (30 Âμg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637015 WB Image
Mouse tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637015 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637015 IHC-P Image
IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637015 IHC-P Image
IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637015 ICC/IF Image
IP3 Receptor I antibody [HL1541] detects IP3 Receptor I protein at endoplasmic reticulum by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: IP3 Receptor I stained by IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:250.Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637015 WB Image
Rat tissue extract (50 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with IP3 Receptor I antibody [HL1541] (GTX637015) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for IP3 Receptor I antibody [HL1541] (GTX637015). Be the first to share your publications with this product.
REVIEW
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