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LAMP1 antibody [GT25212]

KO/KD Comparison Orthogonal
Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336
Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

Cat. No. GTX634336

Reference 5

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT25212

Isotype

 IgG2b

Application

 WB, ICC/IF, IHC-Fr

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all LAMP1 products
PRODUCT

Summary

LAMP1 antibody detects lysosome-associated membrane protein 1 (LAMP-1, or CD107a), a transmembrane glycoprotein that generally migrates at ~120 kDa on western blot. LAMP1 and LAMP2 are major protein components of lysosomal membranes, though both proteins appear to have a more dynamic distribution that includes endosomes, autolysosomes, and multivesicular bodies. Nevertheless, LAMP1 is often used as an indicator of lysosomes in cells.

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

HeLa,HeLa membrane extract

Predict Reactivity

Chimpanzee(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human LAMP1. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888439

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

lysosomal associated membrane protein 1 , CD107a , LAMPA , LGP120

Cellular Localization

Cell membrane; Single-pass type I membrane protein , Endosome membrane; Single-pass type I membrane protein , Lysosome membrane; Single-pass type I membrane protein

Background

The protein encoded by this gene is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. It may also play a role in tumor cell metastasis. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-LAMP1 antibody [GT25212] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634336

GTX634336 ICC/IF Image

LAMP1 antibody [GT25212] detects LAMP1 protein at lysosome by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: LAMP1 stained by LAMP1 antibody [GT25212] (GTX634336) diluted at 1:2000.
Red: alpha Tubulin 4a, a cytoskeleton marker, stained by alpha Tubulin 4a antibody (GTX112141) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

KO/KD

Wild-type (WT) and LAMP1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:10000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

Comparison Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody. (WCE: whole cell extract; ME: membrane extract)

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-LAMP1 antibody [GT25212] used in Western Blot (WB). GTX634336

GTX634336 WB Image

Orthogonal

HeLa whole cell and membrane extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with LAMP1 antibody [GT25212] (GTX634336) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Lin NY et al. Oncogenesis 2022; 11 (1) : 8 C1GALT1 expression predicts a favorable prognosis and suppresses malignant phenotypes via TrkA signaling in neuroblastoma.
Application : ICC/IF
Ryskalin L et al. Nutrients 2021; 13 (10) Lactoferrin Protects against Methamphetamine Toxicity by Modulating Autophagy and Mitochondrial Status.
Application : ICC/IF
Lin DS et al. Cells 2020; 9 (5) Impairment of Proteasome and Autophagy Underlying the Pathogenesis of Leukodystrophy.
Application : IHC-Fr
Fararjeh AS et al. Cancers (Basel) 2019; 11 (4) Proteasome 26S Subunit, non-ATPase 3 (PSMD3) Regulates Breast Cancer by Stabilizing HER2 from Degradation.
Application : WB
Reactivity : Human
Zhang Y et al. Nat Commun 2018; 9 (1) : 4176 Long non-coding RNA CCRR controls cardiac conduction via regulating intercellular coupling.
Application : ICC/IF
Submit a Reference

REVIEW

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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations