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MVP/LRP antibody

KO/KD Orthogonal
Anti-MVP/LRP antibody used in Western Blot (WB). GTX113601
Anti-MVP/LRP antibody used in IHC (Paraffin sections) (IHC-P). GTX113601
Anti-MVP/LRP antibody used in Western Blot (WB). GTX113601
Anti-MVP/LRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113601
Anti-MVP/LRP antibody used in IHC (Paraffin sections) (IHC-P). GTX113601

Cat. No. GTX113601

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all MVP/LRP products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

99 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Rat, Bovine, Cat, Dog(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human MVP/LRP. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037362

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

major vault protein , LRP , VAULT1

Cellular Localization

Cytoplasm , Nucleus , nuclear pore complex

Background

This gene encodes the major vault protein which is a lung resistance-related protein. Vaults are multi-subunit structures that may be involved in nucleo-cytoplasmic transport. This protein mediates drug resistance, perhaps via a transport process. It is widely distributed in normal tissues, and overexpressed in multidrug-resistant cancer cells. The protein overexpression is a potentially useful marker of clinical drug resistance. This gene produces two transcripts by using two alternative exon 2 sequences; however, the open reading frames are the same in both transcripts. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-MVP/LRP antibody used in Western Blot (WB). GTX113601

GTX113601 WB Image

KO/KD

Wild-type (WT) and MVP/LRP knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MVP/LRP antibody (GTX113601) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-MVP/LRP antibody used in IHC (Paraffin sections) (IHC-P). GTX113601

GTX113601 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 xenograft, using LRP(MVP)(GTX113601) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-MVP/LRP antibody used in Western Blot (WB). GTX113601

GTX113601 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MVP/LRP antibody (GTX113601) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-MVP/LRP antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113601

GTX113601 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed A431, using MVP/LRP(GTX113601) antibody at 1:200 dilution.

Anti-MVP/LRP antibody used in IHC (Paraffin sections) (IHC-P). GTX113601

GTX113601 IHC-P Image

MVP/LRP antibody detects MVP/LRP protein at cytoplasm in human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
MVP/LRP antibody (GTX113601) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

REFERENCE

Application Reference

Rayo J et al. Proc Natl Acad Sci U S A 2021; 118 (12) Immunoediting role for major vault protein in apoptotic signaling induced by bacterial N-acyl homoserine lactones.
Application : WB
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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169