APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:10000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Product Note
This antibody is specific for human OGDH protein, and it does not cross react with human OGDHL protein.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant fragment of human OGDH.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
oxoglutarate dehydrogenase , AKGDH , E1k , OGDC
Cellular Localization
Mitochondrion matrix,Nucleus
Background
This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Sep 2009]
Database
Research Area
DATA IMAGES
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/templates/default/js/lightbox/images/loading.gif "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
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GTX637980 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX637980 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637980 WB Image
Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637980 ICC/IF Image
OGDH antibody [HL2072] detects OGDH protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: OGDH stained by OGDH antibody [HL2072] (GTX637980) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637980 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with OGDH antibody [HL2072] (GTX637980) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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REFERENCE
There are currently no references for OGDH antibody [HL2072] (GTX637980). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for OGDH antibody [HL2072] (GTX637980). Be the first to share your experience with this product.
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240223_WB_TPM_watermark_24022619_584.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240223_WB_M_tissue_24022619_323.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240223_WB_R_muscle_24022619_216.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20230317_ICC_IF_24030600_347.webp "Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/small/GTX637980/GTX637980_T-44893_20240202_WB_multiple_B_24021917_358.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240223_WB_TPM_watermark_24022619_584.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240223_WB_M_tissue_24022619_323.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240223_WB_R_muscle_24022619_216.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")
![Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20230317_ICC_IF_24030600_347.webp "Anti-OGDH antibody [HL2072] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637980")
![Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980](/upload/website/prouct_img/normal/GTX637980/GTX637980_T-44893_20240202_WB_multiple_B_24021917_358.webp "Anti-OGDH antibody [HL2072] used in Western Blot (WB). GTX637980")