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PPAR alpha antibody

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX28934
Anti-PPAR alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28934
Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934
Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX28934
Anti-PPAR alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28934
Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934
Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934
Anti-PPAR alpha antibody used in ELISA (ELISA). GTX28934
Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX28934
Anti-PPAR alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28934
Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934

Cat. No. GTX28934

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, FACS, ELISA, ChIP assay

Reactivity

 Human, Mouse, Rat
Package
100 μg ($589)
See all PPAR alpha products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:2000
ICC/IF 1-5 μg/mL
IHC-P 1:100-:300
FACS Assay dependent
ELISA 1:75000-1:125000
ChIP assay Assay dependent
Not tested in other applications.

Calculated MW

52 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide corresponding to a N-Terminal region near amino acids 1-25 of mouse PPAR alpha.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

RRID

AB_372526

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

peroxisome proliferator activated receptor alpha , 4933429D07Rik , AW742785 , Nr1c1 , PPAR-alpha , PPARalpha , Ppar

Cellular Localization

Nucleus

Background

The Peroxisome Proliferator-Activated Receptors (PPARs) are members of the nuclear hormone receptor subfamily of transcription factors. PPARs form heterodimers with retinoid X receptors (RXRs) and these heterodimers regulate transcription of various genes.

Database

Research Area

DATA IMAGES

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX28934

GTX28934 IHC-P Image

Immunohistochemistry showing GeneTex's PPAR alpha antibody staining of PPAR alpha protein in mouse liver tissue section (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before enzymatic antigen retrieval with 0.05% protease in PBS for 5 minutes. Sample was then blocked with 5% serum for 20 minutes at 20ºC. The primary antibody was diluted 1:50 and incubated with sample in Tris plus 5% normal goat serum for 1 hour at 20ºC. A biotinylated goat polyclonal to rabbit IgG was used at dilution at 1:500 as secondary antibody. Images show nuclear staining in hepatocytes (perfusion-fixed mouse, 10 and 40x microscope magnification).

Anti-PPAR alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28934

GTX28934 ICC/IF Image

Immunofluorescence Microscopy of Rabbit anti-PPAR alpha antibody (GTX28934). Tissue: HepG2 cells. Fixation: 4% formaldehyde fixed (10 min). Antigen retrieval: not required. Primary antibody: PPAR alpha antibody at 1 μg/mL overnight at 4ºC. Secondary antibody: Alexa FluorR 488 goat anti-rabbit IgG (H+L) (green) used at a 1:1000, Alexa FluorR 594 WGA was used to label plasma membranes (red) at a 1:200 dilution for 1h for 45 min at RT. Localization: PPAR alpha is nuclear and occasionally cytoplasmic. Staining: PPAR alpha as green fluorescent signal with DAPI (blue) nuclear counterstain.

Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934

GTX28934 WB Image

Affinity Purified anti-PPAR alpha (N -terminal specific) (Rabbit) is shown to detect a 52 kDa band corresponding to PPAR alpha present in a 3T3 whole cell lysate. Approximately 20 μg of lysate was loaded per lane for SDS-PAGE. Detection occurred after using a 1:500 (lane 1) or 1:1000 (lane 2) dilution of antibody followed by 1:2000 dilution of HRP Goat-a-Rabbit IgG for visualization.

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX28934

GTX28934 IHC-P Image

Immunohistochemistry using GeneTex's anti-PPAR antibody, showing staining of PPAR alpha in rat brain sections, highlighting cytoplasmic staining in ependymal cells and neurons in frontal cortex. Bottom image shows subventricular zone (svz) of lateral ventrical (exit point of progenitor olfactory neurones); top image shows frontal cortex in the same section. Cytoplasmic staining is also observed in the corpus callosum (bottom image) and in dendritic fields of the cortex. Formalin/PFA-fixed paraffin-embedded sections of rat brain tissue were incubated with the primary antibody at 1:200 for 1 hour. Antigen retrieval was performed by heat induction in citrate buffer pH 6.0.

Anti-PPAR alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28934

GTX28934 ICC/IF Image

ICC/IF analysis of various MeOH-fixed cells using GTX28934 PPAR alpha antibody.
Panel A : NIH-3T3 cells
Panel B : 293T cells
Panel C : NIH-3T3 cells with secondary antibody only
Green : Primary antibody
Blue : DAPI
Dilution : 10 μg/mL

Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934

GTX28934 WB Image

WB analysis of NIH-3T3 whole cell lysate using GTX28934 PPAR alpha antibody.
Loading : 10 μg
Dilution : 1:1000

Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934

GTX28934 WB Image

WB analysis of NIH-3T3 whole cell lysate using GTX28934 PPAR alpha antibody.
Lane 1 : 1:500
Lane 2 : 1:1000
Loading : 20 μg

Anti-PPAR alpha antibody used in ELISA (ELISA). GTX28934

GTX28934 ELISA Image

ELISA analysis of BSA-conjugated immunizing peptide using serially diluted GTX28934 PPAR alpha antibody.
Coating : 0.1 μg

Anti-PPAR alpha antibody used in IHC (Paraffin sections) (IHC-P). GTX28934

GTX28934 IHC-P Image

IHC-P analysis of mouse liver tissue using GTX28934 PPAR alpha antibody.
Antigen retrieval : 0.05% protease in PBS
Dilution : 1:50

Anti-PPAR alpha antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX28934

GTX28934 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX28934 PPAR alpha antibody.
Green : Primary antibody
Red : Plasma membrane
Blue : DAPI
Dilution : 1 μg/mL

Anti-PPAR alpha antibody used in Western Blot (WB). GTX28934

GTX28934 WB Image

WB analysis of various samples using GTX28934 PPAR alpha antibody.
Lane 1 : 293T whole cell lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : MCF-7 whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lane 5 : A431 whole cell lysate
Lane 6 : LNCaP whole cell lysate
Lane 7 : A-172 whole cell lysate
Lane 8 : NIH-3T3 whole cell lysate
Loading : 35 μg
Dilution : 1 μg/mL

REFERENCE

Application Reference

Cizkova K et al. Biology (Basel) 2022; 11 (7) Lipid Messenger Phosphatidylinositol-4,5-Bisphosphate Is Increased by Both PPARα Activators and Inhibitors: Relevance for Intestinal Cell Differentiation.
Application : ICC/IF
Reactivity : Human
Cizkova K et al. Biomedicines 2021; 9 (9) When Activator and Inhibitor of PPARα Do the Same: Consequence for Differentiation of Human Intestinal Cells.
Application : ICC/IF, IHC-P, ELISA
Reactivity : Human
Nehmi VA et al. J Integr Med 2021; A novel supplement with yeast β-glucan, prebiotic, minerals and Silybum marianum synergistically modulates metabolic and inflammatory pathways and improves steatosis in obese mice.
Application : WB
Reactivity : Mouse
Kuo MW et al. J Biomed Sci 2021; 28 (1) : 7 Yulink, predicted from evolutionary analysis, is involved in cardiac function.
Application : WB
Reactivity : Mouse
Cizkova K et al. Toxicol Appl Pharmacol 2018; 355 : 156-163 Expression of cytochrome P450 epoxygenases and soluble epoxide hydrolase is regulated by hypolipidemic drugs in dose-dependent manner.
Application : ICC/IF
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 589