Perilipin 1 antibody [GT2781]

Name: Perilipin 1 antibody [GT2781]
Brand: GeneTex
SKU: GTX634406
Price: 399.00 USD
Rating: 4 (1 reviews)

Anti-Perilipin 1 antibody [GT2781] used in Western Blot (WB). GTX634406

Anti-Perilipin 1 antibody [GT2781] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX634406

Anti-Perilipin 1 antibody [GT2781] used in IHC (Paraffin sections) (IHC-P). GTX634406

Cat. No. GTX634406

Host	Mouse
Clonality	Monoclonal
Clone Name	GT2781
Isotype	IgG1
Application	WB, ICC/IF, IHC-P
Reactivity	Human, Mouse
Package	100 μl ($399), 25 μl ($169)

See all Perilipin 1 products

Datasheet
Document

View product citations for antibody GTX634406 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent

Not tested in other applications.

Calculated MW

56 kDa. ( Note )

Positive Control

mouse adipose

Predict Reactivity

Rat, Sheep, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Perilipin 1. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888446

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

perilipin 1 , FPLD4 , PERI , PLIN

Cellular Localization

Lipid droplet

Background

The protein encoded by this gene coats lipid storage droplets in adipocytes, thereby protecting them until they can be broken down by hormone-sensitive lipase. The encoded protein is the major cAMP-dependent protein kinase substrate in adipocytes and, when unphosphorylated, may play a role in the inhibition of lipolysis. Alternatively spliced transcript variants varying in the 5' UTR, but encoding the same protein, have been found for this gene. [provided by RefSeq, Feb 2009]

Database

Gene ID: 5346 PLIN1
UniProt: O60240 PLIN1

Research Area

DATA IMAGES

GTX634406 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin 1 antibody [GT2781] (GTX634406) diluted at 1:2000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX634406 ICC/IF Image

Perilipin 1 antibody [GT2781] detects Perilipin 1 protein at lipid droplet by immunofluorescent analysis.
Sample: Mock and differentiated 3T3-L1 cells were fixed in ice-cold MeOH for 5 min.
Green: Perilipin 1 stained by Perilipin 1 antibody [GT2781] (GTX634406) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
The observed subcellular location is based on the publication: PMID: 36204273

GTX634406 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Perilipin antibody [GT2781] (GTX634406) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX634406 IHC-P Image

Perilipin 1 antibody [GT2781] detects Perilipin 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse white adipocyte.
Perilipin 1 stained by Perilipin 1 antibody [GT2781] (GTX634406) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW