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RPS6 antibody

Anti-RPS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113542
Anti-RPS6 antibody used in Western Blot (WB). GTX113542
Anti-RPS6 antibody used in Western Blot (WB). GTX113542
Anti-RPS6 antibody used in Western Blot (WB). GTX113542
Anti-RPS6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113542
Anti-RPS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113542

Cat. No. GTX113542

Reference 6

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IHC-Wm

Reactivity

 Human, Mouse, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all RPS6 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

Mouse brain , HeLa , HepG2

Predict Reactivity

Rat, Drosophila, Bovine, Dog, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.34 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human RPS6. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2037923

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

ribosomal protein S6 , S6

Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a cytoplasmic ribosomal protein that is a component of the 40S subunit. The protein belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-RPS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113542

GTX113542 ICC/IF Image

RPS6 antibody detects RPS6 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RPS6 stained by RPS6 antibody (GTX113542) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-RPS6 antibody used in Western Blot (WB). GTX113542

GTX113542 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 antibody (GTX113542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPS6 antibody used in Western Blot (WB). GTX113542

GTX113542 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPS6 antibody (GTX113542) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPS6 antibody used in Western Blot (WB). GTX113542

GTX113542 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX113542 diluted at 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-RPS6 antibody used in IHC (Paraffin sections) (IHC-P). GTX113542

GTX113542 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using RPS6(GTX113542) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-RPS6 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113542

GTX113542 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed A431, using RPS6(GTX113542) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.

REFERENCE

Application Reference

Westerich KJ et al. bioRxiv 2023; Spatial organization and function of RNA molecules within phase-separated condensates are controlled by Dnd1.
Zhao X et al. Front Cell Neurosci 2022; 16 : 1021592 Huntingtin exon 1 deletion does not alter the subcellular distribution of huntingtin and gene transcription in mice.
Ayoung Kim et al. J Cachexia Sarcopenia Muscle 2021; Ablation of USP21 in skeletal muscle promotes oxidative fibre phenotype, inhibiting obesity and type 2 diabetes.
Application : WB
Reactivity : Mouse
Yeh YH et al. Oncol Rep. 2016; 35 Rhapontigenin inhibits TGF-β-mediated epithelial-mesenchymal transition via the PI3K/AKT/mTOR pathway and is not associated with HIF-1α degradation.
Application : WB
Reactivity : Human
Chen CH et al. Oncotarget 2014; (Epub) Cdc6 cooperates with c-Myc to promote genome instability and EMT in zebrafish.
Application : IHC-Wm
Reactivity : Zebrafish
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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations