Strep epitope Tag antibody [HL1252]

Cat. No. GTX636654

Host	Rabbit
Clonality	Monoclonal
Clone Name	HL1252
Isotype	IgG
Application	WB, ICC/IF, IP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

See all Strep epitope Tag products

Datasheet

View product citations for antibody GTX636654 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000-1:10000
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to Strep epitope Tag.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX636654 ICC/IF Image

Strep epitope Tag antibody [HL1252] detects Strep epitope Tag protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1252] (GTX636654) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX636654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [HL1252] (GTX636654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636654 IP Image

Immunoprecipitation of Strep epitope Tag protein from 293T whole cell extracts using 5 μg of Strep epitope Tag antibody [HL1252] (GTX636654).
Western blot analysis was performed using Strep epitope Tag antibody [HL1252] (GTX636654).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

REFERENCE

There are currently no references for Strep epitope Tag antibody [HL1252] (GTX636654). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for Strep epitope Tag antibody [HL1252] (GTX636654). Be the first to share your experience with this product.

Submit a Review

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty Custom Conjugations

Strep epitope Tag antibody [HL1252]

Cat. No. GTX636654

Host

Clonality

Clone Name

Isotype

Application

Reactivity