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TDP43 antibody [GT733]

KO/KD
Anti-TDP43 antibody [GT733] used in IHC (Paraffin sections) (IHC-P). GTX630197
Anti-TDP43 antibody [GT733] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630197
Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197
Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197
Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197
Anti-TDP43 antibody [GT733] used in IHC (Paraffin sections) (IHC-P). GTX630197
Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197

Cat. No. GTX630197

Reference 1
3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT733

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TDP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Unconjugated

RRID

AB_2888199

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TAR DNA binding protein , ALS10 , TDP-43

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-TDP43 antibody [GT733] used in IHC (Paraffin sections) (IHC-P). GTX630197

GTX630197 IHC-P Image

TDP43 antibody [GT733] detects TDP43 protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 antibody [GT733] (GTX630197) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT733] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630197

GTX630197 ICC/IF Image

TDP43 antibody [GT733] detects TDP43 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDP43 stained by TDP43 antibody [GT733] (GTX630197) diluted at 1:500.
Red: beta Tubulin, a cytoskeleton marker, stained by beta Tubulin antibody (GTX101279) diluted at 1:1000.

Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197

GTX630197 WB Image

KO/KD

Wild-type (WT) and TARDBP knockout (KO) HAP1 cell extracts (50 Âμg) were separated by gradient gel% SDS-PAGE, and the membrane was blotted with TDP43 antibody [GT733] (GTX630197) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.

Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197

GTX630197 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TARDBP antibody [GT733] (GTX630197) diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.

Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197

GTX630197 WB Image

KO/KD

TARDBP antibody [GT733] validation by siRNA knock-down.
Upperpanel: TARDBP antibody [GT733] GTX630197
Lower panel: GAPDH antibody (GTX100118)
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of TARDBP shRNA-transfected 293T cells
10 % SDS-PAGE
TARDBP antibody [GT733] (GTX630197) dilution: 1:1000
GAPDH antibody (GTX100118) dilution: 1:10000

Anti-TDP43 antibody [GT733] used in IHC (Paraffin sections) (IHC-P). GTX630197

GTX630197 IHC-P Image

TDP43 antibody [GT733] detects TDP43 protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 antibody [GT733] (GTX630197) diluted at 1:200.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197

GTX630197 WB Image

TARDBP antibody [GT733] detects TARDBP protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
10 % SDS-PAGE
TARDBP antibody [GT733] (GTX630197) dilution: 1:1000

REFERENCE

Application Reference

Worrall D et al. F1000Res 2023; 12 : 277 The identification of high-performing antibodies for TDP-43 for use in Western Blot, immunoprecipitation and immunofluorescence.
Submit a Reference

REVIEW

TDP43 antibody [GT733] Cat. No. GTX630197

Rating
( Average 5 based on 3 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Immunoprecipitation(IP)
( Average 5 based on 1 users reviews)
Anti-TDP43 antibody [GT733] used in Immunoprecipitation (IP). GTX630197
Date : Anonymous submitted on 09-Nov-2022
Score :
Application Tested : IP
Sample Species : Hu
Sample : HAP1 cells
Lane Description : SM=4% starting material UB=4% unbound fraction IP=immunoprecipitate.
Figure Note : Antibody-bead conjugates were prepared by adding 2.0 μg of antibody to 500 μL of IP Lysis Buffer, together with 30 μL of protein A-Sepharose beads. One ml aliquots at 1.0 mg/ml of lysate were incubated with an antibody-bead conjugate for ~2 hrs at 4ºC. Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-TDP43 antibody [GT733] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX630197
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : ICC/IF
Sample Species : Hu
Sample : HAP1 WT and TARDBP KO cells
Blocking : 5% BSA, 5% goat serum and 0.01% Triton X-100 in PBS, 30min, RT
Fixation : 4% PFA in PBS, 15min, RT
Permeabilization /Antigen retrieval : 0.1% Triton X-100 in PBS, 10min, RT
Primary Antibody : 1:1000, 4°C, > 12Hr
Lane Description :
WT cells: Yellow dashed line
KO cells: Magenta dashed line
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
Anti-TDP43 antibody [GT733] used in Western Blot (WB). GTX630197
Date : Anonymous submitted on 26-Jul-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HAP1 WT and TARDBP KO cells
Amount used : 50μg
Blocking : 5% milk, 1 hr
Primary Antibody : 1:500 in 5% BSA and TBST, 4°C, > 12Hr
Figure Note : Data provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins using knockout technology.
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SDS
PBS.pdf
Package List Price ($)
$ 399
$ 169
Custom Conjugations