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TDP43 antibody

Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in Western Blot (WB). GTX114210
Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210
Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210

Cat. No. GTX114210

Reference 7

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P, IP, ChIP-seq, RIP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all TDP43 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:1000
ChIP-seq Assay dependent
RIP Assay dependent

Note :

IP
target validated by Mass Spectrometry
Not tested in other applications.

Calculated MW

45 kDa. ( Note )

Positive Control

Neuro2A , GL261 , NIH-3T3 , BCL-1 , Raw264.7 , PC-12 , Rat-2

Predict Reactivity

Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human TDP43. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2038095

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

TAR DNA binding protein , ALS10 , TDP-43

Cellular Localization

Nucleus

Background

HIV-1, the causative agent of acquired immunodeficiency syndrome (AIDS), contains an RNA genome that produces a chromosomally integrated DNA during the replicative cycle. Activation of HIV-1 gene expression by the transactivator Tat is dependent on an RNA regulatory element (TAR) located downstream of the transcription initiation site. The protein encoded by this gene is a transcriptional repressor that binds to chromosomally integrated TAR DNA and represses HIV-1 transcription. In addition, this protein regulates alternate splicing of the CFTR gene. A similar pseudogene is present on chromosome 20. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-TDP43 antibody used in Immunoprecipitation (IP). GTX114210

GTX114210 IP Image

TARDBP antibody immunoprecipitates TARDBP protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A. 40 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of TARDBP protein by 2 μg of TARDBP antibody (GTX114210) 12% SDS-PAGE The immunoprecipitated TARDBP protein was detected by TARDBP antibody (GTX114210) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 Xenograft , using TDP-43(GTX114210) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210

GTX114210 ICC/IF Image

TDP43 antibody detects TDP43 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:2000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

TARDBP antibody detects TARDBP protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
D. 30 μg BCL-1 whole cell lysate/extract
E. 30 μg Raw264.7 whole cell lysate/extract
10% SDS-PAGE
TARDBP antibody (GTX114210) dilution: 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in Western Blot (WB). GTX114210

GTX114210 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with TDP43 antibody (GTX114210) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-TDP43 antibody used in IHC (Paraffin sections) (IHC-P). GTX114210

GTX114210 IHC-P Image

TDP43 antibody detects TDP43 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-TDP43 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX114210

GTX114210 ICC/IF Image

TDP43 antibody detects TDP43 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: TDP43 stained by TDP43 antibody (GTX114210) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

REFERENCE

Application Reference

Harrell TL et al. Int J Mol Sci 2023; 24 (3) Herpes Simplex Virus 1 (HSV-1) Infected Cell Protein 0 (ICP0) Targets of Ubiquitination during Productive Infection of Primary Adult Sensory Neurons.
Application : WB
Reactivity : Mouse
Chu JF et al. Cell Rep 2019; 29 (10) : 3118-3133.e6 TDP-43 Regulates Coupled Dendritic mRNA Transport-Translation Processes in Co-operation with FMRP and Staufen1.
Application : ICC/IF, RIP
Reactivity : Mouse
Wu LS et al. Acta Neuropathol Commun 2019; 7 (1) : 50 Transcriptomopathies of pre- and post-symptomatic frontotemporal dementia-like mice with TDP-43 depletion in forebrain neurons.
Application : IHC-P
Reactivity : Mouse
Huang CY et al. Journal of Neuroimmunology 2017; : Epub ahead of print TDP-43 proteolysis is associated with astrocyte reactivity after traumatic brain injury in rodents.
Application : IHC-P
Majumder P et al. Acta Neuropathol 2016; 132 (5) : 721-738 Co-regulation of mRNA translation by TDP-43 and Fragile X Syndrome protein FMRP.
Application : WB, ICC/IF, IP
Reactivity : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169