V5 tag antibody [GT1078]

Cat. No. GTX635260

Host	Mouse
Clonality	Monoclonal
Clone Name	GT1078
Isotype	IgG2a
Application	WB, ICC/IF, IP
Reactivity	Species independent
Package	100 μl ($399), 25 μl ($169)

See all V5 tag products

Datasheet
Document

View product citations for antibody GTX635260 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.99 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to V5 tag

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888514

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Research Area

DATA IMAGES

GTX635260 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with V5 Tag antibody [GT1078] (GTX635260) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX635260 ICC/IF Image

V5 tag antibody [GT1078] detects V5 tag protein by immunofluorescent analysis.
Sample: V5-tag tagged protein transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: V5 tag stained by V5 tag antibody [GT1078] (GTX635260) diluted at 1:2000.
Blue: Hoechst 33342 staining.

GTX635260 IP Image

Immunoprecipitation of V5 tag protein from transfected 293T using 10 μg of V5 tag antibody [GT1078] (GTX635260).
Western blot analysis was performed using V5 tag antibody (GTX117997).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01).

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf

Package

List Price ($)

100 μl

$ 399

25 μl

$ 169

Qty

V5 tag antibody [GT1078]

Cat. No. GTX635260

Host

Clonality

Clone Name

Isotype

Application

Reactivity