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Yellow Fever virus NS4B Protein antibody [HL2449]

Recombinant antibody
Anti-Yellow Fever virus NS4B Protein antibody [HL2449] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638767
Anti-Yellow Fever virus NS4B Protein antibody [HL2449] used in Western Blot (WB). GTX638767

Cat. No. GTX638767

1

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2449

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Yellow Fever virus
Package
100 μl ($399),
25 μl ($169)
See all Yellow Fever virus NS4B Protein products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Yellow Fever virus

Immunogen

Full length Yellow Fever virus NS4B Protein.(strain 17D vaccine)

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Database

Research Area

DATA IMAGES

Anti-Yellow Fever virus NS4B Protein antibody [HL2449] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638767

GTX638767 ICC/IF Image

Yellow Fever virus NS4B Protein antibody [HL2449] detects Yellow Fever virus NS4B Protein protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Yellow Fever virus NS4B Protein stained by Yellow Fever virus NS4B Protein antibody [HL2449] (GTX638767) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Yellow Fever virus NS4B Protein antibody [HL2449] used in Western Blot (WB). GTX638767

GTX638767 WB Image

Yellow Fever Virus viral lysate (0.1 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Yellow Fever virus NS4B Protein antibody [HL2449] (GTX638767) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

Yellow Fever virus NS4B Protein antibody [HL2449] Cat. No. GTX638767

Rating
( Average 5 based on 1 users reviews)
Application
Immunocytochemistry/ Immunofluorescence(ICC/IF)
( Average 5 based on 1 users reviews)
Anti-Yellow Fever virus NS4B Protein antibody [HL2449] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638767
Date : Anonymous submitted on 15-Feb-2024
Score :
Application Tested : ICC/IF
Sample : VeroE6 cells with/without ZIKV virus infection (MOI 0.1, 24hr)
Blocking : PBS, 2% BSA, 0.5Hr
Fixation : 4 % formal- dehyde
Permeabilization /Antigen retrieval : PBS, 0.5 % Triton X-100, 20 mM glycine
Primary Antibody : 1:1000, °C, Hr
Figure Note :
Orange : Primary antibody
Blue : DAPI
Images were acquired using a Leica SP8 Laser Scanning Confocal Microscope with a 63x oil objective (Leica) and Leica Application Suit X software.
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Package List Price ($)
$ 399
$ 169
Custom Conjugations