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alpha Amylase antibody (HRP)

Anti-alpha Amylase antibody (HRP) used in Western Blot (WB). GTX40567
Anti-alpha Amylase antibody (HRP) used in Western Blot (WB). GTX40567

Cat. No. GTX40567

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Bacillus amyloliquefaciens
Package
1 mg ($399)
See all alpha Amylase products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:5000
IP 1:100
ELISA 1:5000-1:20000
Not tested in other applications.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl, 1% BSA

Preservative

0.01% Gentamicin Sulfate

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

10 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bacillus amyloliquefaciens

Immunogen

Bacillus amyloliquefaciens a-Amylase protein.

Purification

IgG fraction
This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Conjugation

Horseradish peroxidase(HRP)

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Background

Amylase catalyses the hydrolysis of internal 1,4-glucan links in large linear polymers at internal bonds. The enzyme is present in all living organisms with variable enzymatic activity.

Research Area

DATA IMAGES

Anti-alpha Amylase antibody (HRP) used in Western Blot (WB). GTX40567

GTX40567 WB Image

Western Blot of anti-Alpha Amylase antibody.(HRP) Load: 100 ng Alpha Amylase per lane. Blocking for 30 min at RT. Predicted/Observed size: 57 kDa for Alpha Amylase.

Anti-alpha Amylase antibody (HRP) used in Western Blot (WB). GTX40567

GTX40567 WB Image

WB analysis of Bacillus amyloliquefaciens alpha amylase protein using GTX40567 alpha Amylase antibody (HRP).
Loading : 100 ng
Dilution : 1:1000

REFERENCE

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REVIEW

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Package List Price ($)
$ 399