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alpha Smooth Muscle Actin antibody [1A4] (FITC)

Cat. No. GTX72531

Reference 12

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 1A4

Isotype

 IgG2a

Application

 ICC/IF, IHC-P, IHC-Fr, FACS

Reactivity

 Human, Mouse, Rat, Rabbit, Guinea pig, Baboon
Package
100 μg ($399)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

For IF: Use at 10-20 μg/ml for 2 hours at room temperature. For IF: paraffin-embedded or frozen sections, as well as on cell smears can be used. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

42 kDa. ( Note )

Positive Control

Bowel

Product Note

This antibody reacts with the alpha-smooth muscle isoform of actin. It does not react with actin from fibroblasts (beta- and gamma-cytoplasmic), striated muscle (alpha-sarcomeric) and myocardium (alpha-myocardial).

PROPERTIES

Form

Liquid

Buffer

50mM Potassium Phosphate pH7.2, 150mM Sodium Chloride, 10mg/ml BSA, carrier polysaccharides

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE. Protect from light.

Antigen Species

Human

Immunogen

The N-terminal decapeptide of alpha-smooth muscle actin

Purification

Unpurified

Conjugation

Fluorescein isothiocyanate (FITC)
_x000B_Absorption : 495 nm_x000B_ ; Emission : 528 nm._x000B_ Ratio : 0.7 molecules FITC per Mouse IgG molecule.

RRID

AB_383662

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , ACTSA

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

REFERENCE

Application Reference

P Bisaccia et al. Stem Cells Transl Med 2023; : szad070 Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia
Zhou Z et al. Adv Sci (Weinh) 2023; 10 (23) : e2300548 A T Cell-Engaging Tumor Organoid Platform for Pancreatic Cancer Immunotherapy.

Clone Reference

S Kecsk?s et al. Front Aging Neurosci 2023; 15 (1175281) Nimodipine augments cerebrovascular reactivity in aging but runs the risk of local perfusion reduction in acute cerebral ischemia
Layton TB et al. Nat Commun 2020; 11 (1):2768 Cellular census of human fibrosis defines functionally distinct stromal cell types and states
Application : FACS
Reactivity : Human
Varga DP et al. Neurobiol Dis 2020; 137:104780 The antagonism of prostaglandin FP receptors inhibits the evolution of spreading depolarization in an experimental model of global forebrain ischemia.
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REVIEW

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Application Reference
P Bisaccia et al. Stem Cells Transl Med 2023;:szad070 Extracellular Vesicles From Mesenchymal Umbilical Cord Cells Exert Protection Against Oxidative Stress and Fibrosis in a Rat Model of Bronchopulmonary Dysplasia
Zhou Z et al. Adv Sci (Weinh) 2023; 10 (23):e2300548 A T Cell-Engaging Tumor Organoid Platform for Pancreatic Cancer Immunotherapy.
Medley SC et al. Wound Repair Regen 2020; Fibroblast-specific Stat1 deletion enhances the myofibroblast phenotype during tissue repair.
Application : IHC-P
Reactivity : Mouse
Aslani F et al. Hum Reprod 2015; 30 (2):417-31 Targeting high mobility group box protein 1 ameliorates testicular inflammation in experimental autoimmune orchitis.
Akishima-Fukasawa Y et al. Am J Clin Pathol 2010; 134 (1):26177 Significance of PGP9.5 expression in cancer-associated fibroblasts for prognosis of colorectal carcinoma.
Backhouse S et al. Invest Ophthalmol Vis Sci 2010; 51 (12):6162-71 Effect of induced myopia on scleral myofibroblasts and in vivo ocular biomechanical compliance in the guinea pig.
Application : IHC-Fr
Reactivity : Guinea pig
Clone Reference
S Kecsk?s et al. Front Aging Neurosci 2023; 15 (1175281) Nimodipine augments cerebrovascular reactivity in aging but runs the risk of local perfusion reduction in acute cerebral ischemia
Layton TB et al. Nat Commun 2020; 11 (1):2768 Cellular census of human fibrosis defines functionally distinct stromal cell types and states
Application : FACS
Reactivity : Human
Varga DP et al. Neurobiol Dis 2020; 137:104780 The antagonism of prostaglandin FP receptors inhibits the evolution of spreading depolarization in an experimental model of global forebrain ischemia.
Li DJ et al. Redox Biol 2018; 15:22-23 Cholinergic anti-inflammatory pathway inhibits neointimal hyperplasia by suppressing inflammation and oxidative stress.
Gorchs L et al. Front Oncol 2015; 5:87 Cancer-Associated Fibroblasts from Lung Tumors Maintain Their Immunosuppressive Abilities after High-Dose Irradiation.
Application : FACS
Reactivity : Human
Wan W et al. Cardiovasc Res 2015; 106 (3):478-87 Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.
Application : IHC-Fr
Reactivity : Mouse
SDS
Sodium Azide.pdf
Package List Price ($)
$ 399