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alpha Smooth Muscle Actin antibody [HL1419]

Recombinant antibody Orthogonal
Anti-alpha Smooth Muscle Actin antibody [HL1419] used in IHC (Paraffin sections) (IHC-P). GTX636885
Anti-alpha Smooth Muscle Actin antibody [HL1419] used in IHC (Paraffin sections) (IHC-P). GTX636885
Anti-alpha Smooth Muscle Actin antibody [HL1419] used in Western Blot (WB). GTX636885
Anti-alpha Smooth Muscle Actin antibody [HL1419] used in IHC (Paraffin sections) (IHC-P). GTX636885

Cat. No. GTX636885

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1419

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all alpha Smooth Muscle Actin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human alpha Smooth Muscle Actin. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin alpha 2, smooth muscle , ACTSA

Cellular Localization

Cytoplasm, cytoskeleton

Background

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, integrity, and intercellular signaling. The encoded protein is a smooth muscle actin that is involved in vascular contractility and blood pressure homeostasis. Mutations in this gene cause a variety of vascular diseases, such as thoracic aortic disease, coronary artery disease, stroke, and Moyamoya disease, as well as multisystemic smooth muscle dysfunction syndrome. [provided by RefSeq, Sep 2017]

Database

  • Gene ID: 59 ACTA2
  • UniProt: P62736 ACTA2

Research Area

DATA IMAGES

Anti-alpha Smooth Muscle Actin antibody [HL1419] used in IHC (Paraffin sections) (IHC-P). GTX636885

GTX636885 IHC-P Image

alpha Smooth Muscle Actin antibody [HL1419] detects alpha Smooth Muscle Actin protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL1419] (GTX636885) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody [HL1419] used in IHC (Paraffin sections) (IHC-P). GTX636885

GTX636885 IHC-P Image

alpha Smooth Muscle Actin antibody [HL1419] detects alpha Smooth Muscle Actin protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL1419] (GTX636885) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-alpha Smooth Muscle Actin antibody [HL1419] used in Western Blot (WB). GTX636885

GTX636885 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Smooth Muscle Actin antibody [HL1419] (GTX636885) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Smooth Muscle Actin antibody [HL1419] used in IHC (Paraffin sections) (IHC-P). GTX636885

GTX636885 IHC-P Image

alpha Smooth Muscle Actin antibody [HL1419] detects alpha Smooth Muscle Actin protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse smooth muscle.
alpha Smooth Muscle Actin stained by alpha Smooth Muscle Actin antibody [HL1419] (GTX636885) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations