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alpha Tubulin antibody [GT114] (HRP)

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01
Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01
Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01
Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

Cat. No. GTX628802-01

Reference 126

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 GT114

Isotype

 IgG2a

Application

 WB

Reactivity

 Human, Mouse, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all alpha Tubulin products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
Not tested in other applications.

Calculated MW

50 kDa. ( Note )

Positive Control

Jurkat , Raji , NCI-H929

PROPERTIES

Form

Liquid

Buffer

0.05M Tris, 0.15M NaCl, 1%BSA

Preservative

0.005% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

Purification

Affinity purified by Protein G.

Conjugation

Horseradish peroxidase(HRP)

RRID

AB_2888054

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

K-ALPHA-1 antibody , TUBA1B antibody , alpha-tubulin ubiquitous antibody , alpha tubulin antibody , tubulin K-alpha-1 antibody , tubulin alpha-ubiquitous chain antibody , tubulin , alpha , ubiquitous antibody , tubulin alpha-1B chain antibody , tubulin , alpha 1b antibody

Background

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.

Research Area

DATA IMAGES

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-alpha Tubulin antibody [GT114] (HRP) used in Western Blot (WB). GTX628802-01

GTX628802-01 WB Image

alpha Tubulin antibody [GT114] (HRP) detects alpha Tubulin protein by Western blot analysis.
A. 30 μg Jurkat whole cell lysate/extract
B. 30 μg Raji whole cell lysate/extract
C. 30 μg NCI-H929 whole cell lysate/extract
10 % SDS-PAGE
alpha Tubulin antibody [GT114] (HRP) (GTX628802-01) dilution: 1:10000

REFERENCE

Application Reference

Waller V et al. Front Oncol 2023; 13 : 1117326 The role of EphA2 in ADAM17- and ionizing radiation-enhanced lung cancer cell migration.
Application : WB

Clone Reference

Tsugami Y et al. J Mammary Gland Biol Neoplasia 2023; 28 (1):3 Valine Treatment Enhances Antimicrobial Component Production in Mammary Epithelial Cells and the Milk of Lactating Goats Without Influencing the Tight Junction Barrier.
Moon SJ et al. Cancers (Basel) 2023; 15 (4) CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer.
Lu H.A. et al. Circ Res. 2022; 130 (1):112-129 Alternative Splicing Mediated by RNA-Binding Protein RBM24 Facilitates Cardiac Myofibrillogenesis in a Differentiation Stage-Specific Manner.
Li Y et al. Bioengineered 2022; 13 (1):800-809 Sitagliptin ameliorates hypoxia-induced damages in endometrial stromal cells: an implication in endometriosis.
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REVIEW

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Application Reference
Waller V et al. Front Oncol 2023; 13:1117326 The role of EphA2 in ADAM17- and ionizing radiation-enhanced lung cancer cell migration.
Application : WB
Monta??s-Agudo P et al. J Cell Sci 2021; Inhibition of minor intron splicing reduces Na+ and Ca2+ channel expression and function in cardiomyocytes.
Application : WB
Reactivity : Human
Clone Reference
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Moon SJ et al. Cancers (Basel) 2023; 15 (4) CTTN Overexpression Confers Cancer Stem Cell-like Properties and Trastuzumab Resistance via DKK-1/WNT Signaling in HER2 Positive Breast Cancer.
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